plenti-puro-GSDMD WT
(Plasmid
#249728)
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PurposeExpress GSDMD WT in mammalian cells with lentiviurs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249728 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti puro
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti-puro-GSDMD WT was a gift from Hao Wu (Addgene plasmid # 249728 ; http://n2t.net/addgene:249728 ; RRID:Addgene_249728) -
For your References section:
ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D. Du G, Healy LB, David L, Walker C, El-Baba TJ, Lutomski CA, Goh B, Gu B, Pi X, Devant P, Fontana P, Dong Y, Ma X, Miao R, Balasubramanian A, Puthenveetil R, Banerjee A, Luo HR, Kagan JC, Oh SF, Robinson CV, Lieberman J, Wu H. Nature. 2024 Jun;630(8016):437-446. doi: 10.1038/s41586-024-07373-5. Epub 2024 Apr 10. 10.1038/s41586-024-07373-5 PubMed 38599239