Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24989||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namea wild type human MAP1-LC3B fused with EYFP
SpeciesH. sapiens (human)
Insert Size (bp)378
Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
/ Fusion Protein
- EYFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl2 (not destroyed)
- 3′ cloning site SalI/XhoI (destroyed during cloning)
- 5′ sequencing primer GFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEX-YFP-hLC3WT was a gift from Isei Tanida (Addgene plasmid # 24989 ; http://n2t.net/addgene:24989 ; RRID:Addgene_24989)
For your References section:Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K. Autophagy. 2008 Jan 1. 4(1):131-4. PubMed 18000393
Map uploaded by the depositor.