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pQint
(Plasmid #25819)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25819 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAT19
  • Backbone size w/o insert (bp) 6559
  • Vector type
    bacterial gene inactivation
  • Selectable markers
    erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    erythromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    replicates in Clostridium phytofermentans and E.coli with 200ug/ml erm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    qQint
  • Insert Size (bp)
    3609
  • Mutation
    plasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQint was a gift from George Church (Addgene plasmid # 25819)
  • For your References section:

    Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Tolonen AC, Chilaka AC, Church GM. Mol Microbiol. 2009 Dec . 74(6):1300-13. 10.1111/j.1365-2958.2009.06890.x PubMed 19775243