|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25819||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6559
Vector typebacterial gene inactivation
Growth in Bacteria
Growth instructionsreplicates in Clostridium phytofermentans and E.coli with 200ug/ml erm
Copy numberHigh Copy
Insert Size (bp)3609
Mutationplasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQint was a gift from George Church (Addgene plasmid # 25819 ; http://n2t.net/addgene:25819 ; RRID:Addgene_25819)
For your References section:Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Tolonen AC, Chilaka AC, Church GM. Mol Microbiol. 2009 Dec . 74(6):1300-13. 10.1111/j.1365-2958.2009.06890.x PubMed 19775243