pQint
(Plasmid #25819)

• Depositing Lab: George Church
• Publication: Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Backbone

• Vector backbone: pAT19
• Backbone size w/o insert (bp): 6559
• Vector type: bacterial gene inactivation
• Selectable markers: erythromycin

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: replicates in Clostridium phytofermentans and E.coli with 200ug/ml erm
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: qQint
• Insert Size (bp): 3609
• Mutation: plasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern.