Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #25819)


Item Catalog # Description Quantity Price (USD)
Plasmid 25819 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6559
  • Vector type
    bacterial gene inactivation
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    replicates in Clostridium phytofermentans and E.coli with 200ug/ml erm
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)
  • Mutation
    plasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQint was a gift from George Church (Addgene plasmid # 25819 ; ; RRID:Addgene_25819)
  • For your References section:

    Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Tolonen AC, Chilaka AC, Church GM. Mol Microbiol. 2009 Dec . 74(6):1300-13. 10.1111/j.1365-2958.2009.06890.x PubMed 19775243