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pQexp
(Plasmid #25820)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25820 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAT19
  • Backbone size w/o insert (bp) 6559
  • Vector type
    Bacterial Expression
  • Selectable markers
    erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    erythromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    replicates in Clostridium phytofermentans and E.coli with 200 ug/ml erm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pQexp
  • Insert Size (bp)
    413

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site pstI (not destroyed)
  • 3′ cloning site bamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene sequence has a small insertion and small deletion compared to author sequence. These differences are in regions of the plasmid backbone that were not modified and should not affect function of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQexp was a gift from George Church (Addgene plasmid # 25820)
  • For your References section:

    Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Tolonen AC, Chilaka AC, Church GM. Mol Microbiol. 2009 Dec . 74(6):1300-13. 10.1111/j.1365-2958.2009.06890.x PubMed 19775243