pQexp
(Plasmid #25820)

• Depositing Lab: George Church
• Publication: Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Backbone

• Vector backbone: pAT19
• Backbone size w/o insert (bp): 6559
• Vector type: Bacterial Expression
• Selectable markers: erythromycin

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: replicates in Clostridium phytofermentans and E.coli with 200 ug/ml erm
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pQexp
• Insert Size (bp): 413

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: pstI (not destroyed)
• 3′ cloning site: bamHI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequence has a small insertion and small deletion compared to author sequence. These differences are in regions of the plasmid backbone that were not modified and should not affect function of this plasmid.