|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25820||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6559
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsreplicates in Clostridium phytofermentans and E.coli with 200 ug/ml erm
Copy numberHigh Copy
Insert Size (bp)413
- Cloning method Restriction Enzyme
- 5′ cloning site pstI (not destroyed)
- 3′ cloning site bamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Addgene sequence has a small insertion and small deletion compared to author sequence. These differences are in regions of the plasmid backbone that were not modified and should not affect function of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQexp was a gift from George Church (Addgene plasmid # 25820 ; http://n2t.net/addgene:25820 ; RRID:Addgene_25820)
For your References section:Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Tolonen AC, Chilaka AC, Church GM. Mol Microbiol. 2009 Dec . 74(6):1300-13. 10.1111/j.1365-2958.2009.06890.x PubMed 19775243