Skip to main content
Holiday Schedule: Addgene will be closed December 24 - December 31. Order processing and shipping will resume on January 3, 2022. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET28-mE1 Citations (9)

Plasmid Article: High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1.
Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sa-Miranda C, Azevedo JE Mol Biotechnol. 2011 Oct 20.
PubMed Journal

Articles Citing pET28-mE1

A Rapid and Versatile Method for Generating Proteins with Defined Ubiquitin Chains. Martinez-Fonts K, Matouschek A. Biochemistry. 2016 Mar 29;55(12):1898-908. doi: 10.1021/acs.biochem.5b01310. Epub 2016 Mar 17. PubMed
Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome. Reichard EL, Chirico GG, Dewey WJ, Nassif ND, Bard KE, Millas NE, Kraut DA. J Biol Chem. 2016 Aug 26;291(35):18547-61. doi: 10.1074/jbc.M116.720151. Epub 2016 Jul 12. PubMed
UbFluor: A Fluorescent Thioester to Monitor HECT E3 Ligase Catalysis. Krist DT, Foote PK, Statsyuk AV. Curr Protoc Chem Biol. 2017 Mar 2;9(1):11-37. doi: 10.1002/cpch.17. PubMed
Structural Studies of HHARI/UbcH7 approximately Ub Reveal Unique E2 approximately Ub Conformational Restriction by RBR RING1. Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA, Klevit RE. Structure. 2017 Jun 6;25(6):890-900.e5. doi: 10.1016/j.str.2017.04.013. Epub 2017 May 25. PubMed
An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome. Worden EJ, Dong KC, Martin A. Mol Cell. 2017 Sep 7;67(5):799-811.e8. doi: 10.1016/j.molcel.2017.07.023. Epub 2017 Aug 24. PubMed
Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions. Dao TP, Kolaitis RM, Kim HJ, O'Donovan K, Martyniak B, Colicino E, Hehnly H, Taylor JP, Castaneda CA. Mol Cell. 2018 Mar 15;69(6):965-978.e6. doi: 10.1016/j.molcel.2018.02.004. Epub 2018 Mar 8. PubMed
Enzymatic Assembly of Ubiquitin Chains. Michel MA, Komander D, Elliott PR. Methods Mol Biol. 2018;1844:73-84. doi: 10.1007/978-1-4939-8706-1_6. PubMed
The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize Substrate Degradation. Bard JAM, Bashore C, Dong KC, Martin A. Cell. 2019 Apr 4;177(2):286-298.e15. doi: 10.1016/j.cell.2019.02.031. Epub 2019 Mar 28. PubMed
Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils. Morimoto D, Nishizawa R, Walinda E, Takashima S, Sugase K, Shirakawa M. Polymers (Basel). 2018 Feb 27;10(3). pii: polym10030240. doi: 10.3390/polym10030240. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.