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pET28-mE1 Citations (15)

Originally described in: High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1.
Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sa-Miranda C, Azevedo JE Mol Biotechnol. 2011 Oct 20.
PubMed Journal

Articles Citing pET28-mE1

A Rapid and Versatile Method for Generating Proteins with Defined Ubiquitin Chains. Martinez-Fonts K, Matouschek A. Biochemistry. 2016 Mar 29;55(12):1898-908. doi: 10.1021/acs.biochem.5b01310. Epub 2016 Mar 17. PubMed
Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome. Reichard EL, Chirico GG, Dewey WJ, Nassif ND, Bard KE, Millas NE, Kraut DA. J Biol Chem. 2016 Aug 26;291(35):18547-61. doi: 10.1074/jbc.M116.720151. Epub 2016 Jul 12. PubMed
UbFluor: A Fluorescent Thioester to Monitor HECT E3 Ligase Catalysis. Krist DT, Foote PK, Statsyuk AV. Curr Protoc Chem Biol. 2017 Mar 2;9(1):11-37. doi: 10.1002/cpch.17. PubMed
Structural Studies of HHARI/UbcH7 approximately Ub Reveal Unique E2 approximately Ub Conformational Restriction by RBR RING1. Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA, Klevit RE. Structure. 2017 Jun 6;25(6):890-900.e5. doi: 10.1016/j.str.2017.04.013. Epub 2017 May 25. PubMed
An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome. Worden EJ, Dong KC, Martin A. Mol Cell. 2017 Sep 7;67(5):799-811.e8. doi: 10.1016/j.molcel.2017.07.023. Epub 2017 Aug 24. PubMed
Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions. Dao TP, Kolaitis RM, Kim HJ, O'Donovan K, Martyniak B, Colicino E, Hehnly H, Taylor JP, Castaneda CA. Mol Cell. 2018 Mar 15;69(6):965-978.e6. doi: 10.1016/j.molcel.2018.02.004. Epub 2018 Mar 8. PubMed

Associated Plasmids

Enzymatic Assembly of Ubiquitin Chains. Michel MA, Komander D, Elliott PR. Methods Mol Biol. 2018;1844:73-84. doi: 10.1007/978-1-4939-8706-1_6. PubMed
The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize Substrate Degradation. Bard JAM, Bashore C, Dong KC, Martin A. Cell. 2019 Apr 4;177(2):286-298.e15. doi: 10.1016/j.cell.2019.02.031. Epub 2019 Mar 28. PubMed
Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils. Morimoto D, Nishizawa R, Walinda E, Takashima S, Sugase K, Shirakawa M. Polymers (Basel). 2018 Feb 27;10(3). pii: polym10030240. doi: 10.3390/polym10030240. PubMed
Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses. Banchenko S, Krupp F, Gotthold C, Burger J, Graziadei A, O'Reilly FJ, Sinn L, Ruda O, Rappsilber J, Spahn CMT, Mielke T, Taylor IA, Schwefel D. PLoS Pathog. 2021 Aug 2;17(8):e1009775. doi: 10.1371/journal.ppat.1009775. eCollection 2021 Aug. PubMed
Ubiquitin-directed AAA+ ATPase p97/VCP unfolds stable proteins crosslinked to DNA for proteolysis by SPRTN. Kroning A, van den Boom J, Kracht M, Kueck AF, Meyer H. J Biol Chem. 2022 Jun;298(6):101976. doi: 10.1016/j.jbc.2022.101976. Epub 2022 Apr 22. PubMed
The Ufd1 cofactor determines the linkage specificity of polyubiquitin chain engagement by the AAA+ ATPase Cdc48. Williams C, Dong KC, Arkinson C, Martin A. Mol Cell. 2023 Mar 2;83(5):759-769.e7. doi: 10.1016/j.molcel.2023.01.016. Epub 2023 Feb 2. PubMed
Structure of the human UBR5 E3 ubiquitin ligase. Wang F, He Q, Zhan W, Yu Z, Finkin-Groner E, Ma X, Lin G, Li H. Structure. 2023 May 4;31(5):541-552.e4. doi: 10.1016/j.str.2023.03.010. Epub 2023 Apr 10. PubMed
Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing. Williams C, Dong KC, Arkinson C, Martin A. STAR Protoc. 2023 Dec 15;4(4):102659. doi: 10.1016/j.xpro.2023.102659. Epub 2023 Oct 26. PubMed
Nedd4-2-dependent regulation of astrocytic Kir4.1 and Connexin43 controls neuronal network activity. Altas B, Rhee HJ, Ju A, Solis HC, Karaca S, Winchenbach J, Kaplan-Arabaci O, Schwark M, Ambrozkiewicz MC, Lee C, Spieth L, Wieser GL, Chaugule VK, Majoul I, Hassan MA, Goel R, Wojcik SM, Koganezawa N, Hanamura K, Rotin D, Pichler A, Mitkovski M, de Hoz L, Poulopoulos A, Urlaub H, Jahn O, Saher G, Brose N, Rhee J, Kawabe H. J Cell Biol. 2024 Jan 1;223(1):e201902050. doi: 10.1083/jcb.201902050. Epub 2023 Nov 30. PubMed

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