Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pAM1580
(Plasmid #40241)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40241 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    1573
  • Total vector size (bp) 10806
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    promoterless luxAB reporter vector
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
    2400
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NSII vector

Lux Genes: luxAB

cloning sites: NheI, XhoI, SmaI, XbaI, StuI, and SalI.

luxAB as SalI-PvuII from pLAVI (pAM884) into EcoRV-SalI cut pAM1573. SalI, StuI, XbaI, SmaI, XhoI, NheI still unique. Glows in E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1580 was a gift from Susan Golden (Addgene plasmid # 40241 ; http://n2t.net/addgene:40241 ; RRID:Addgene_40241)
  • For your References section:

    Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624