|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40241||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 10806
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Gene/Insert namepromoterless luxAB reporter vector
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)2400
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Lux Genes: luxAB
cloning sites: NheI, XhoI, SmaI, XbaI, StuI, and SalI.
luxAB as SalI-PvuII from pLAVI (pAM884) into EcoRV-SalI cut pAM1573. SalI, StuI, XbaI, SmaI, XhoI, NheI still unique. Glows in E. coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1580 was a gift from Susan Golden (Addgene plasmid # 40241 ; http://n2t.net/addgene:40241 ; RRID:Addgene_40241)
For your References section:Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624