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Depositing Lab
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Depositing OrganizationETH Zurich (Swiss Federal Institute of Technology)
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40571-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40571-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRES-puro2b
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5125
- Total vector size (bp) 7504
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAurora B FRET-sensor N-terminally fused to histone 2B
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Alt nameH2B-CyPet-FHA2-Aurora B substrate-YPet
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Alt namechromatin-targeted Aurora B biosensor
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SpeciesSynthetic
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Insert Size (bp)2379
- Promoter CMV
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Tags
/ Fusion Proteins
- CyPet (N terminal on insert)
- YPet (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMichael Lampson, Department of Biology, University of Pennsylvania, Philadelphia, US
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 40571-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40571-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 40571 ; http://n2t.net/addgene:40571 ; RRID:Addgene_40571) -
For your References section:
Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW. J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16. 10.1083/jcb.201112112 PubMed 22801782
Map uploaded by the depositor.
