pEGFP Cep170 C-term (Nigg GG2)
(Plasmid #41151)

• Depositing Lab: Erich Nigg
• Depositing Organization: University of Basel
  Located in Switzerland
• Publication: Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107. Epub 2004 Dec 22.
• Citations: 1 Article

Backbone

• Vector backbone: pEGFP-T7/C1 (modified pEGFP-C1)
• Backbone manufacturer: Modified from Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7400
• Modifications to backbone: From pEGFP-C1: T7 promoter inserted into the vector NheI site
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cep170
• Alt name: Centrosomal protein 170kDa
• Alt name: KIAA0470
• Alt name: FAM68A
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Mutation: Contains C-terminal fragment (aa 755-1460, from XmnI site to the end of cDNA),
• GenBank ID: AB007939
• Entrez Gene: CEP170 (a.k.a. FAM68A, KAB, KIAA0470)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: KIAA0470 cDNA obtained from the Kazusa DNA Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The KIAA0470 cDNA was obtained from the Kazusa DNA Research Institute. It was subcloned into pT7-EGFP-C1 (modified from pEGFP-C1; BD Biosciences Clontech), after removing the ATG. Specifically, a 5kb SalI-SnaBI (Klenow blunted) fragment from pBS/KIAA0470Δ5'UTR was ligated into pT7-EGFP-C1 (Nigg COM81) digested with XhoI (Klenow blunted). The 3' XhoI site was recreated. This resulted in Addgene plasmid #41150: pEGFP Cep170 (Nigg PID200). The plasmid was then digested with BspEI and XmnI, blunted, and religated to recover a plasmid containing only the Cep170 C-terminal portion (aa 755-1460, from XmnI site to the end of cDNA).

DNA (Catalog # 41151-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 41151-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.