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pCW57.1 Notes

Addgene Diagnostic Digest for 41393

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 41393 with the following enzymes: EcoRI-HF single digest, KpnI-HF single digest, and AgeI-HF + KpnI-HF double digest. The expected pattern of bands was observed.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pCW57.1 with the following enzymes: EcoRI-HF and KpnI-HF.

Expected Bands:
EcoRI-HF 9354 bp
EcoRI-HF/ KpnI-HF 5527 bp & 3827 bp

The results show the expected pattern, but there may be a faint band around 1.6 kb. This band is possibly due to recombination of the large, lentiviral vector. While we do not expect this band is a concern for plasmid function, one could linearize the vector, gel purify the large, linear band, and ligate to remove the recombinant band.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pCW57.1 with the following enzymes: NotI-HF.

Expected Bands:
NotI-HF 7.9 kb & 1.5 kb

These results show the expected pattern.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pCW57.1with the following enzymes: EcoRI-HF and NdeI.

Expected Bands
EcoRI-HF/ NdeI: 6301 bp & 3053 bp

We observe the expected bands for this digest.