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(Plasmid #45188)


Item Catalog # Description Quantity Price (USD)
Plasmid 45188 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pLenti-CaMKIIa-eArch 3.0-EYFP (Addgene plasmid# 35514)
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 9239
  • Total vector size (bp) 10793
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use Amp at 50ug/mL
  • Copy number
    High Copy


  • Gene/Insert name
    enhanced Archaerhodopsin
  • Alt name
  • Species
    Synthetic; H. sodomense
  • Insert Size (bp)
  • Mutation
    D95Q, D106E
  • Promoter CamKIIa
  • Tag / Fusion Protein
    • eYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ctgacgaaggctcgcgaggc
  • 3′ sequencing primer gccatacgggaagcaatagcatg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Arch-EEQ was a gift from Mark Schnitzer (Addgene plasmid # 45188 ; ; RRID:Addgene_45188)
  • For your References section:

    Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators. Gong Y, Li JZ, Schnitzer MJ. PLoS One. 2013 Jun 19;8(6):e66959. 10.1371/journal.pone.0066959 PubMed 23840563