pLPS-mOct4-gfp
(Plasmid #49394)

• Purpose: mammalian expression of mouse Oct3/4 fused to EGFP
• Depositing Lab: Randall McKinnon
• Publication: McKinnon Lab plasmids (unpublished)

Backbone

• Vector backbone: pLPS-3'EGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression, Cre/Lox ; acceptor vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct4
• Alt name: Pou5f1
• Alt name: POU domain, class 5, transcription factor 1
• Alt name: Oct3/4
• Species: M. musculus (mouse)
• Insert Size (bp): 1039
• Mutation: 1101 (tga to ccc) with respect to GenBank ID BC068268
• GenBank ID: BC068268
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mOct4 was transferred from the donor vector (pDNR-mOct3/4, Addgene plasmid #49393) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to mOct4, through intron splicing.