pJ404-GST-DJ1
(Plasmid
#51490)
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PurposeInducible expression of human DJ1 in E.coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepJExpress404-T5-amp-High
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Backbone manufacturerDNA2.0
- Backbone size w/o insert (bp) 3986
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDJ1
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SpeciesH. sapiens (human)
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Insert Size (bp)1248
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Entrez GenePARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
- Promoter T5
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Tag
/ Fusion Protein
- GST-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T5
- 3′ sequencing primer rrnB-T1-term-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthesized and cloned by DNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJ404-GST-DJ1 was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 51490)