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M2371 his3::ADE2 Disruptor Converter
(Plasmid #51671)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51671 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJJ215
  • Total vector size (bp) 7639
  • Vector type
    Yeast Expression ; yeast marker swap
  • Selectable markers
    ADE2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADE2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ADE2 (a.k.a. YOR128C)
  • Promoter ADE2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Plasmid was made by cutting pJJ215 with MscI and Nsi I and replacing the 570 bp MscI–Nsi I fragment with a 3.6 kb NotI (blunted with Klenow)–PstI fragment containing ADE2 from plasmid M1144. Plasmid M1144 contains a 3.6 kb BglII fragment (partial digest product) with ADE2 with BamHI linkers cloned into the BamHI site of Bluescript KS+.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M2371 his3::ADE2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51671 ; http://n2t.net/addgene:51671 ; RRID:Addgene_51671)
  • For your References section:

    New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713