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PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
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Depositing Lab
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Depositing OrganizationWashington University in Saint Louis
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 59018-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 59018-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAT-GFP
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Backbone manufacturerBoris Striepen
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7500
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Modifications to backboneunknown
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Vector typeToxoplasma Expression
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Selectable markerschloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameYFP-TetR
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Alt nameTetracycline Repressor Fused to Yellow Fluorescent Protein
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SpeciesToxoplasma gondii
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Insert Size (bp)1500
- Promoter alpha-tubulin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site avrII (unknown if destroyed)
- 3′ cloning site pstI (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 59018-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 59018-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAT-YFP-TetR was a gift from David Sibley (Addgene plasmid # 59018 ; http://n2t.net/addgene:59018 ; RRID:Addgene_59018) -
For your References section:
The Toxoplasma pseudokinase ROP5 forms complexes with ROP18 and ROP17 kinases that synergize to control acute virulence in mice. Etheridge RD, Alaganan A, Tang K, Lou HJ, Turk BE, Sibley LD. Cell Host Microbe. 2014 May 14;15(5):537-50. doi: 10.1016/j.chom.2014.04.002. 10.1016/j.chom.2014.04.002 PubMed 24832449
