PurposeBicistronic Lentivirus expressing sox2 and Cre
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59019||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneGFP-Cre Empty vector
- Backbone size w/o insert (bp) 11000
- Total vector size (bp) 12000
Modifications to backboneB-actin promoter replaces the Ubc promoter, Sox2 replaces the GFP
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesM. musculus (mouse)
Insert Size (bp)960
Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
- Promoter B-actin
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer gcgtcggtcgggctgcaacc
- 3′ sequencing primer CAGCGGGGCTGCTAAAGCGCATGC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byADDGENE PLASMID #13367 WAS USED AS TEMPLATE FOR CLONING OF SOX2
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ACTIN-SOX2-PGK Cre was a gift from Trudy Oliver (Addgene plasmid # 59019 ; http://n2t.net/addgene:59019 ; RRID:Addgene_59019)
For your References section:Sox2 Cooperates with Lkb1 Loss in a Mouse Model of Squamous Cell Lung Cancer. Mukhopadhyay A, Berrett KC, Kc U, Clair PM, Pop SM, Carr SR, Witt BL, Oliver TG. Cell Rep. 2014 Jul 10;8(1):40-49. doi: 10.1016/j.celrep.2014.05.036. Epub 2014 Jun 19. 10.1016/j.celrep.2014.05.036 PubMed 24953650
Map uploaded by the depositor.