gRNA-hIRF-1 #12/pSIR
(Plasmid #61080)

• Purpose: Expresses a guide RNA (gRNA) to target human IRF-1 promoter
• Depositing Lab: Hodaka Fujii
• Publication: Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014.

Backbone

• Vector backbone: pSIR
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8083
• Total vector size (bp): 8638
• Vector type: Mammalian Expression, Retroviral, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA_hIRF1 promoter #12
• Species: H. sapiens (human)
• Insert Size (bp): 555
• GenBank ID: DQ789232.1
• Entrez Gene: IRF1 (a.k.a. IMD117, IRF-1, MAR)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: ATACTGGCCGTTCTCCTCTTCTGA
• 3′ sequencing primer: GCGCTTACACTTTAGGAGACACTC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

gRNA target sequence: CCGGGGGCGCTGGGCTGTCCCGG

For more information on Fujii Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/fujii/