MG1655-tMMR
(Bacterial strain #62392)

• Purpose: E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression
• Depositing Lab: Csaba Pál
• Publication: Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. doi: 10.1093/nar/gku105. Epub 2014 Feb 5.

Backbone

• Vector backbone: genomic
• Selectable markers: TET

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): K12 MG1655
• Growth instructions: We recommend that it be streaked on an LB plate containing 15 µg/ml tetracycline at 30°C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MG1655-tMMR
• Alt name: MGλ-tMMR
• Species: E. coli
• Mutation: mutS(A134V) mutL(G62S); nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]}
• Promoter: n/a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The heat shock-inducible genomic λ Red system was P1 transduced from E. coli LT521 {MG1655 gal490 nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} (courtesy of Donald L. Court, National Cancer Institute, Frederick, MD, USA).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For detailed use and MAGE, see:

1. Nyerges, Á. et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucl. Acids Res. 42, e62–e62 (2014).

2. Gallagher, R. R., Li, Z., Lewis, A. O. & Isaacs, F. J. Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat. Protocols 9, 2301–2316 (2014).

3. Bonde, M. T., Anderson, M. V., Wallin, A. I. N., Wang, H. H. & Sommer, M. O. A. MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucl. Acids Res. (2014). doi:10.1093/nar/gku428