p5E-acta2
(Plasmid #78686)

• Purpose: acta2 promoter for Gateway cloning
• Depositing Lab: Sarah Childs
• Publication: Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014.

Backbone

• Vector backbone: pDONRp4p1r
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2600
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: acta2 promoter
• Alt name: actin, alpha 2, smooth muscle, aorta
• Alt name: alpha smooth muscle actin
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2165
• GenBank ID: NM_212620.1
• Entrez Gene: acta2 (a.k.a. wu:fb63d03)
• Promoter: acta2

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The entire intron 1 was inserted upstream of 300 bp of proximal acta2 promoter to make a compact promoter/enhancer construct that could easily be used for transgenesis.