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Addgene

pET22b - PA
(Plasmid #84863)

Ordering

This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 84863 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PA
  • Alt name
    protective antigen
  • Species
    Bacillus anthracis
  • Promoter T7
  • Tag / Fusion Protein
    • pelB signal sequence (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b - PA was a gift from Russell Vance (Addgene plasmid # 84863 ; http://n2t.net/addgene:84863 ; RRID:Addgene_84863)
  • For your References section:

    NAIP proteins are required for cytosolic detection of specific bacterial ligands in vivo. Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, Kazmierczak BI, Vance RE. J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub 2016 Apr 4. 10.1084/jem.20151809 PubMed 27045008