PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86666||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
- Backbone size w/o insert (bp) 4821
- Total vector size (bp) 7919
Modifications to backboneadd WPRE to the 3' of the insert
Vector typeMammalian Expression, Mouse Targeting, Lentiviral, Synthetic Biology
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameCANE-LV envelope
Insert Size (bp)1919
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer cttcttctttttcctacagc
- 3′ sequencing primer tttcacaaattttgtaatcc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CANE-LV envelope was a gift from Fan Wang (Addgene plasmid # 86666 ; http://n2t.net/addgene:86666 ; RRID:Addgene_86666)
For your References section:Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit. Sakurai K, Zhao S, Takatoh J, Rodriguez E, Lu J, Leavitt AD, Fu M, Han BX, Wang F. Neuron. 2016 Nov 23;92(4):739-753. doi: 10.1016/j.neuron.2016.10.015. Epub 2016 Oct 27. 10.1016/j.neuron.2016.10.015 PubMed 27974160
Map uploaded by the depositor.