Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pFLEX+mCyclin D1
(Plasmid #86793)


Item Catalog # Description Quantity Price (USD)
Plasmid 86793 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    cyclin D1
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Ccnd1 (a.k.a. CycD1, Cyl-1, PRAD1, bcl-1, cD1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Alan Diehl; when in Drs Sherr and Roussel laboratories

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mouse cyclin D1 wild type was cloned into the EcoRI site of pFLEX vector. In frame EcoRI site of FLAG-Tag mouse cyclin D1 was engineered by PCR and confirmed by sequence analysis. Protein expression confirmed by immunofluorescence and immunoblotting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLEX+mCyclin D1 was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 86793 ; ; RRID:Addgene_86793)
  • For your References section:

    Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle. Matsushime H, Roussel MF, Ashmun RA, Sherr CJ. Cell. 1991 May 17;65(4):701-13. 0092-8674(91)90101-4 [pii] PubMed 1827757