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Addgene

pFLEX+mCyclin D1
(Plasmid #86793)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86793 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFLEX
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D1
  • Alt name
    ccnd1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Ccnd1 (a.k.a. CycD1, Cyl-1, PRAD1, bcl-1, cD1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Alan Diehl; when in Drs Sherr and Roussel laboratories

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mouse cyclin D1 wild type was cloned into the EcoRI site of pFLEX vector. In frame EcoRI site of FLAG-Tag mouse cyclin D1 was engineered by PCR and confirmed by sequence analysis. Protein expression confirmed by immunofluorescence and immunoblotting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLEX+mCyclin D1 was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 86793 ; http://n2t.net/addgene:86793 ; RRID:Addgene_86793)
  • For your References section:

    Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle. Matsushime H, Roussel MF, Ashmun RA, Sherr CJ. Cell. 1991 May 17;65(4):701-13. 0092-8674(91)90101-4 [pii] PubMed 1827757