pRK793 Citations (109)
Originally described in: Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, Copeland TD, Waugh DS Protein Eng. 2001 Dec . 14(12):993-1000. PubMed Journal
Articles Citing pRK793
| Articles |
|---|
| Human glucocorticoid-induced TNF receptor ligand regulates its signaling activity through multiple oligomerization states. Zhou Z, Song X, Berezov A, Zhang G, Li Y, Zhang H, Murali R, Li B, Greene MI. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5465-70. doi: 10.1073/pnas.0711350105. Epub 2008 Mar 31. PubMed |
| Expression, purification and structural characterization of recombinant hepcidin, an antimicrobial peptide identified in Japanese flounder, Paralichthys olivaceus. Srinivasulu B, Syvitski R, Seo JK, Mattatall NR, Knickle LC, Douglas SE. Protein Expr Purif. 2008 Sep;61(1):36-44. doi: 10.1016/j.pep.2008.05.012. Epub 2008 May 29. PubMed |
| Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus. Choi JM, Hutson AM, Estes MK, Prasad BV. Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9175-80. doi: 10.1073/pnas.0803275105. Epub 2008 Jul 2. PubMed |
| Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease. Tubb MR, Smith LE, Davidson WS. J Lipid Res. 2009 Jul;50(7):1497-504. doi: 10.1194/jlr.D900003-JLR200. Epub 2009 Mar 24. PubMed |
| Triazole-linked inhibitors of inosine monophosphate dehydrogenase from human and Mycobacterium tuberculosis. Chen L, Wilson DJ, Xu Y, Aldrich CC, Felczak K, Sham YY, Pankiewicz KW. J Med Chem. 2010 Jun 24;53(12):4768-78. doi: 10.1021/jm100424m. PubMed |
| Vibrational Stark effect spectroscopy at the interface of Ras and Rap1A bound to the Ras binding domain of RalGDS reveals an electrostatic mechanism for protein-protein interaction. Stafford AJ, Ensign DL, Webb LJ. J Phys Chem B. 2010 Nov 25;114(46):15331-44. doi: 10.1021/jp106974e. Epub 2010 Oct 22. PubMed |
| Purification and crystallization of RNase HIII from Staphylococcus aureus. Reiling SA, Homma K, Asojo OA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):79-82. doi: 10.1107/S1744309110045616. Epub 2010 Dec 22. PubMed |
| Electrostatic effects of mutations of Ras glutamine 61 measured using vibrational spectroscopy of a thiocyanate probe. Stafford AJ, Walker DM, Webb LJ. Biochemistry. 2012 Apr 3;51(13):2757-67. doi: 10.1021/bi201225p. Epub 2012 Mar 21. PubMed |
| Expression and purification of recombinant human tyrosine hydroxylase as a fusion protein in Escherichia coli. Higgins CA, Vermeer LM, Doorn JA, Roman DL. Protein Expr Purif. 2012 Aug;84(2):219-23. doi: 10.1016/j.pep.2012.05.007. Epub 2012 Jun 1. PubMed |
| Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis. Lee K, Rhee K. Cell Cycle. 2012 Jul 1;11(13):2476-85. doi: 10.4161/cc.20878. Epub 2012 Jul 1. PubMed |
| Structure and properties of the C-terminal beta-helical domain of VgrG protein from Escherichia coli O157. Uchida K, Leiman PG, Arisaka F, Kanamaru S. J Biochem. 2014 Mar;155(3):173-82. doi: 10.1093/jb/mvt109. Epub 2013 Dec 3. PubMed |
| Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies. Hung YF, Valdau O, Schunke S, Stern O, Koenig BW, Willbold D, Hoffmann S. PLoS One. 2014 Jan 23;9(1):e86482. doi: 10.1371/journal.pone.0086482. eCollection 2014. PubMed |
| Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant. Fang J, Zou L, Zhou X, Cheng B, Fan J. PLoS One. 2014 Nov 26;9(11):e112254. doi: 10.1371/journal.pone.0112254. eCollection 2014. PubMed |
| Structural and functional characterization of the acidic region from the RIZ tumor suppressor. Sun Y, Stine JM, Atwater DZ, Sharmin A, Ross JB, Briknarova K. Biochemistry. 2015 Feb 17;54(6):1390-400. doi: 10.1021/bi501398w. Epub 2015 Feb 2. PubMed |
| Arrestin-3-Dependent Activation of c-Jun N-Terminal Kinases (JNKs). Zhan X, Kook S, Kaoud TS, Dalby KN, Gurevich EV, Gurevich VV. Curr Protoc Pharmacol. 2015 Mar 2;68:2.12.1-26. doi: 10.1002/0471141755.ph0212s68. PubMed |
| Hydrogen bond donation to the heme distal ligand of Staphylococcus aureus IsdG tunes the electronic structure. Lockhart CL, Conger MA, Pittman DS, Liptak MD. J Biol Inorg Chem. 2015 Jul;20(5):757-70. doi: 10.1007/s00775-015-1263-5. Epub 2015 Apr 25. PubMed |
| Rapid large-scale purification of myofilament proteins using a cleavable His6-tag. Zhang M, Martin JL, Kumar M, Khairallah RJ, de Tombe PP. Am J Physiol Heart Circ Physiol. 2015 Nov;309(9):H1509-15. doi: 10.1152/ajpheart.00598.2015. Epub 2015 Sep 18. PubMed |
| Expression and purification of the alpha subunit of the epithelial sodium channel, ENaC. Reddy BG, Dai Q, McNicholas CM, Fuller CM, Kappes JC, DeLucas LJ. Protein Expr Purif. 2016 Jan;117:67-75. doi: 10.1016/j.pep.2015.09.014. Epub 2015 Sep 21. PubMed |
| Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA. Gruss F, Hiller S, Maier T. Methods Mol Biol. 2015;1329:259-70. doi: 10.1007/978-1-4939-2871-2_20. PubMed |
| Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis. Ueda M, Shimosaka M, Arai R. Acta Crystallogr F Struct Biol Commun. 2015 Dec 1;71(Pt 12):1516-20. doi: 10.1107/S2053230X15022001. Epub 2015 Nov 27. PubMed |
| The conserved TPR-containing C-terminal domain of Pseudomonas aeruginosa FimV is required for its cAMP-dependent and independent functions. Buensuceso RN, Nguyen Y, Zhang K, Daniel-Ivad M, Sugiman-Marangos SN, Fleetwood AD, Zhulin IB, Junop MS, Howell PL, Burrows LL. J Bacteriol. 2016 Jun 13. pii: JB.00322-16. PubMed |
| Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro. Naumann C, Mot AC, Dissmeyer N. Methods Mol Biol. 2016;1450:55-83. doi: 10.1007/978-1-4939-3759-2_6. PubMed |
| A Nonsense Mutation in Mycobacterium marinum That Is Suppressible by a Novel Mechanism. Williams EA, Mba Medie F, Bosserman RE, Johnson BK, Reyna C, Ferrell MJ, Champion MM, Abramovitch RB, Champion PA. Infect Immun. 2017 Jan 26;85(2). pii: e00653-16. doi: 10.1128/IAI.00653-16. Print 2017 Feb. PubMed |
T-REX on-demand redox targeting in live cells.
Parvez S, Long MJ, Lin HY, Zhao Y, Haegele JA, Pham VN, Lee DK, Aye Y.
Nat Protoc. 2016 Dec;11(12):2328-2356. doi: 10.1038/nprot.2016.114. Epub 2016 Oct 27.
PubMed
Associated Plasmids |
| Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies. Jain RW, Dang AK, Kerfoot SM. J Vis Exp. 2016 Oct 27;(116). doi: 10.3791/54727. PubMed |
| Mechanically Watching the ClpXP Proteolytic Machinery. Cordova JC, Olivares AO, Lang MJ. Methods Mol Biol. 2017;1486:317-341. PubMed |
| Catalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleTJE. Matthews S, Belcher JD, Tee KL, Girvan HM, McLean KJ, Rigby SE, Levy CW, Leys D, Parker DA, Blankley RT, Munro AW. J Biol Chem. 2017 Jan 4. pii: jbc.M116.762336. doi: 10.1074/jbc.M116.762336. PubMed |
| Akt3 is a privileged first responder in isozyme-specific electrophile response. Long MJ, Parvez S, Zhao Y, Surya SL, Wang Y, Zhang S, Aye Y. Nat Chem Biol. 2017 Mar;13(3):333-338. doi: 10.1038/nchembio.2284. Epub 2017 Jan 23. PubMed |
| Production of alkenes and novel secondary products by P450 OleTJE using novel H2 O2 -generating fusion protein systems. Matthews S, Tee KL, Rattray NJ, McLean KJ, Leys D, Parker DA, Blankley RT, Munro AW. FEBS Lett. 2017 Mar;591(5):737-750. doi: 10.1002/1873-3468.12581. Epub 2017 Feb 21. PubMed |
| Biochemical and biophysical investigations of the interaction between human glucokinase and pro-apoptotic BAD. Rexford A, Zorio DA, Miller BG. PLoS One. 2017 Feb 9;12(2):e0171587. doi: 10.1371/journal.pone.0171587. eCollection 2017. PubMed |
| Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery. Vazquez-Cintron EJ, Beske PH, Tenezaca L, Tran BQ, Oyler JM, Glotfelty EJ, Angeles CA, Syngkon A, Mukherjee J, Kalb SR, Band PA, McNutt PM, Shoemaker CB, Ichtchenko K. Sci Rep. 2017 Feb 21;7:42923. doi: 10.1038/srep42923. PubMed |
Real-time detection of N-end rule-mediated ubiquitination via fluorescently labeled substrate probes.
Mot AC, Prell E, Klecker M, Naumann C, Faden F, Westermann B, Dissmeyer N.
New Phytol. 2017 Mar 9. doi: 10.1111/nph.14497.
PubMed
Associated Plasmids |
| His-Tag-Mediated Dimerization of Chemoreceptors Leads to Assembly of Functional Nanoarrays. Haglin ER, Yang W, Briegel A, Thompson LK. Biochemistry. 2017 Nov 7;56(44):5874-5885. doi: 10.1021/acs.biochem.7b00698. Epub 2017 Sep 22. PubMed |
| Crystal Structure of a Plant Multidrug and Toxic Compound Extrusion Family Protein. Tanaka Y, Iwaki S, Tsukazaki T. Structure. 2017 Sep 5;25(9):1455-1460.e2. doi: 10.1016/j.str.2017.07.009. PubMed |
| Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Shinohara Y, Koyama YM, Ukai-Tadenuma M, Hirokawa T, Kikuchi M, Yamada RG, Ukai H, Fujishima H, Umehara T, Tainaka K, Ueda HR. Mol Cell. 2017 Sep 7;67(5):783-798.e20. doi: 10.1016/j.molcel.2017.08.009. PubMed |
| Biochemical and structural characterization of a novel cooperative binding mode by Pit-1 with CATT repeats in the macrophage migration inhibitory factor promoter. Agarwal S, Cho TY. Nucleic Acids Res. 2018 Jan 25;46(2):929-941. doi: 10.1093/nar/gkx1183. PubMed |
| Further insights into the metal ion binding abilities and the metalation pathway of a plant metallothionein from Musa acuminata. Cabral ACS, Jakovleska J, Deb A, Penner-Hahn JE, Pecoraro VL, Freisinger E. J Biol Inorg Chem. 2018 Jan;23(1):91-107. doi: 10.1007/s00775-017-1513-9. Epub 2017 Dec 7. PubMed |
| Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection. Verma V, Kaur C, Grover P, Gupta A, Chaudhary VK. PLoS One. 2018 Jan 23;13(1):e0191315. doi: 10.1371/journal.pone.0191315. eCollection 2018. PubMed |
| A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes. Uddin I, Frank S, Warren MJ, Pickersgill RW. Small. 2018 May;14(19):e1704020. doi: 10.1002/smll.201704020. Epub 2018 Mar 24. PubMed |
| Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s. Benoit MPMH, Asenjo AB, Sosa H. Nat Commun. 2018 Apr 25;9(1):1662. doi: 10.1038/s41467-018-04044-8. PubMed |
| Leishmania phosphatase PP5 is a regulator of HSP83 phosphorylation and essential for parasite pathogenicity. Norris-Mullins B, Krivda JS, Smith KL, Ferrell MJ, Morales MA. Parasitol Res. 2018 Sep;117(9):2971-2985. doi: 10.1007/s00436-018-5994-4. Epub 2018 Jul 8. PubMed |
| Structural Basis for MARK1 Kinase Autoinhibition by Its KA1 Domain. Emptage RP, Lemmon MA, Ferguson KM, Marmorstein R. Structure. 2018 Aug 7;26(8):1137-1143.e3. doi: 10.1016/j.str.2018.05.008. Epub 2018 Jun 28. PubMed |
| Structural and catalytic properties of the peroxygenase P450 enzyme CYP152K6 from Bacillus methanolicus. Girvan HM, Poddar H, McLean KJ, Nelson DR, Hollywood KA, Levy CW, Leys D, Munro AW. J Inorg Biochem. 2018 Nov;188:18-28. doi: 10.1016/j.jinorgbio.2018.08.002. Epub 2018 Aug 3. PubMed |
| A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc. Habjanic J, Zerbe O, Freisinger E. Metallomics. 2018 Oct 17;10(10):1415-1429. doi: 10.1039/c8mt00193f. PubMed |
| Redox control and autoxidation of class 1, 2 and 3 phytoglobins from Arabidopsis thaliana. Mot AC, Puscas C, Miclea P, Naumova-Letia G, Dorneanu S, Podar D, Dissmeyer N, Silaghi-Dumitrescu R. Sci Rep. 2018 Sep 12;8(1):13714. doi: 10.1038/s41598-018-31922-4. PubMed |
| Mutually Orthogonal Nonsense-Suppression Systems and Conjugation Chemistries for Precise Protein Labeling at up to Three Distinct Sites. Italia JS, Addy PS, Erickson SB, Peeler JC, Weerapana E, Chatterjee A. J Am Chem Soc. 2019 Apr 17;141(15):6204-6212. doi: 10.1021/jacs.8b12954. Epub 2019 Apr 8. PubMed |
| Bismaleimide cross-linked anthrax toxin forms functional octamers with high specificity in tumor targeting. Fischer ES, Campbell WA 4th, Liu S, Ghirlando R, Fattah RJ, Bugge TH, Leppla SH. Protein Sci. 2019 Jun;28(6):1059-1070. doi: 10.1002/pro.3613. Epub 2019 Apr 17. PubMed |
| Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System. Gillette W, Frank P, Perkins S, Drew M, Grose C, Esposito D. Methods Mol Biol. 2019;2009:259-277. doi: 10.1007/978-1-4939-9532-5_20. PubMed |
| Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site. Rosen LE, Klebba JE, Asfaha JB, Ghent CM, Campbell MG, Cheng Y, Morgan DO. Nat Commun. 2019 Nov 15;10(1):5189. doi: 10.1038/s41467-019-13209-y. PubMed |
| Impact of naturally occurring serine/cysteine variations on the structure and function of Pseudomonas metallothioneins. Habjanic J, Chesnov S, Zerbe O, Freisinger E. Metallomics. 2020 Jan 29;12(1):23-33. doi: 10.1039/c9mt00213h. PubMed |
Directed evolution improves the catalytic efficiency of TEV protease.
Sanchez MI, Ting AY.
Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7.
PubMed
Associated Plasmids |
| Wss1 homolog from Candida albicans and its role in DNA-protein crosslink tolerance. Homchan A, Sukted J, Mongkolsuk S, Jeruzalmi D, Matangkasombut O, Pakotiprapha D. Mol Microbiol. 2020 Sep;114(3):409-422. doi: 10.1111/mmi.14518. Epub 2020 May 13. PubMed |
| G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Yang P, Mathieu C, Kolaitis RM, Zhang P, Messing J, Yurtsever U, Yang Z, Wu J, Li Y, Pan Q, Yu J, Martin EW, Mittag T, Kim HJ, Taylor JP. Cell. 2020 Apr 16;181(2):325-345.e28. doi: 10.1016/j.cell.2020.03.046. PubMed |
| Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles. Norris JL, Patel T, Dasari AKR, Cope TA, Lim KH, Hughes RM. J Biotechnol. 2020 Oct 10;322:1-9. doi: 10.1016/j.jbiotec.2020.06.021. Epub 2020 Jun 30. PubMed |
| Metallation pathway of a plant metallothionein: Cicer arietinum MT2. Salim A, Chesnov S, Freisinger E. J Inorg Biochem. 2020 Sep;210:111157. doi: 10.1016/j.jinorgbio.2020.111157. Epub 2020 Jun 24. PubMed |
| Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells. Ti SC, Wieczorek M, Kapoor TM. STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100011. Epub 2020 Jun 3. PubMed |
| Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection. Nguyen LT, Smith BM, Jain PK. Nat Commun. 2020 Sep 30;11(1):4906. doi: 10.1038/s41467-020-18615-1. PubMed |
| Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays. Klein S, Stern D, Seeber F. BMC Biotechnol. 2020 Oct 6;20(1):53. doi: 10.1186/s12896-020-00646-7. PubMed |
| Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. Liu J, Xue Z, Vann KR, Shi X, Kutateladze TG. STAR Protoc. 2020 Oct 28;1(3):100155. doi: 10.1016/j.xpro.2020.100155. eCollection 2020 Dec 18. PubMed |
| Functional and structural characterization of a flavoprotein monooxygenase essential for biogenesis of tryptophylquinone cofactor. Oozeki T, Nakai T, Kozakai K, Okamoto K, Kuroda S, Kobayashi K, Tanizawa K, Okajima T. Nat Commun. 2021 Feb 10;12(1):933. doi: 10.1038/s41467-021-21200-9. PubMed |
| Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task. Becker I, Wang-Eckhardt L, Lodder-Gadaczek J, Wang Y, Grunewald A, Eckhardt M. J Neurochem. 2021 Jun;157(6):2008-2023. doi: 10.1111/jnc.15333. Epub 2021 Mar 17. PubMed |
| Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates. Martinez SE, Singh A, De Wijngaert B, Sultana S, Dharia C, Vanbuel H, Shen J, Vasilchuk D, Patel SS, Das K. STAR Protoc. 2021 Mar 31;2(2):100431. doi: 10.1016/j.xpro.2021.100431. eCollection 2021 Jun 18. PubMed |
| Development of a Broad-Spectrum Antiserum against Cobra Venoms Using Recombinant Three-Finger Toxins. Liu BS, Jiang BR, Hu KC, Liu CH, Hsieh WC, Lin MH, Sung WC. Toxins (Basel). 2021 Aug 10;13(8):556. doi: 10.3390/toxins13080556. PubMed |
| Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies. Cantrell MS, Wall JD, Pu X, Turner M, Woodbury L, Fujise K, McDougal OM, Warner LR. Protein Expr Purif. 2022 Jan;189:105989. doi: 10.1016/j.pep.2021.105989. Epub 2021 Oct 6. PubMed |
| Unlocking SARS-CoV-2 detection in low- and middle-income countries. Alcantara R, Penaranda K, Mendoza-Rojas G, Nakamoto JA, Martins-Luna J, Del Valle-Mendoza J, Adaui V, Milon P. Cell Rep Methods. 2021 Oct 21:100093. doi: 10.1016/j.crmeth.2021.100093. PubMed |
| The TFAMoplex-Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent. Burger M, Kaelin S, Leroux JC. Adv Sci (Weinh). 2022 Mar;9(8):e2104987. doi: 10.1002/advs.202104987. Epub 2022 Jan 17. PubMed |
Compartmentalization of telomeres through DNA-scaffolded phase separation.
Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH, Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP.
Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 10.1016/j.devcel.2021.12.017.
PubMed
Associated Plasmids |
| Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales. Zhao Y, Miranda Herrera PA, Chang D, Hamelin R, Long MJC, Aye Y. Proc Natl Acad Sci U S A. 2022 Feb 1;119(5). pii: 2120687119. doi: 10.1073/pnas.2120687119. PubMed |
| Amyloid conformation-dependent disaggregation in a reconstituted yeast prion system. Nakagawa Y, Shen HC, Komi Y, Sugiyama S, Kurinomaru T, Tomabechi Y, Krayukhina E, Okamoto K, Yokoyama T, Shirouzu M, Uchiyama S, Inaba M, Niwa T, Sako Y, Taguchi H, Tanaka M. Nat Chem Biol. 2022 Mar;18(3):321-331. doi: 10.1038/s41589-021-00951-y. Epub 2022 Feb 17. PubMed |
| Poxviruses and paramyxoviruses use a conserved mechanism of STAT1 antagonism to inhibit interferon signaling. Talbot-Cooper C, Pantelejevs T, Shannon JP, Cherry CR, Au MT, Hyvonen M, Hickman HD, Smith GL. Cell Host Microbe. 2022 Mar 9;30(3):357-372.e11. doi: 10.1016/j.chom.2022.01.014. Epub 2022 Feb 18. PubMed |
| Citrylglutamate synthase deficient male mice are subfertile with impaired histone and transition protein 2 removal in late spermatids. Wang-Eckhardt L, Sylvester M, Becker I, Allam JP, Eckhardt M. Biochem J. 2022 May 13;479(9):953-972. doi: 10.1042/BCJ20210844. PubMed |
| Divergent binding mode for a protozoan BRC repeat to RAD51. Pantelejevs T, Hyvonen M. Biochem J. 2022 May 27;479(10):1031-1043. doi: 10.1042/BCJ20220141. PubMed |
| Effects of Alexander disease-associated mutations on the assembly and organization of GFAP intermediate filaments. Yang AW, Lin NH, Yeh TH, Snider N, Perng MD. Mol Biol Cell. 2022 Jul 1;33(8):ar69. doi: 10.1091/mbc.E22-01-0013. Epub 2022 May 5. PubMed |
| Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditions. Glassey E, King AM, Anderson DA, Zhang Z, Voigt CA. PLoS One. 2022 Sep 19;17(9):e0266488. doi: 10.1371/journal.pone.0266488. eCollection 2022. PubMed |
| Fluorescence dequenching assay for the activity of TEV protease. Paththamperuma C, Page RC. Anal Biochem. 2022 Dec 15;659:114954. doi: 10.1016/j.ab.2022.114954. Epub 2022 Oct 18. PubMed |
| Shelterin reduces the accessibility of telomeric overhangs. Shiekh S, Jack A, Saurabh A, Mustafa G, Kodikara SG, Gyawali P, Hoque ME, Presse S, Yildiz A, Balci H. Nucleic Acids Res. 2022 Dec 9;50(22):12885-12895. doi: 10.1093/nar/gkac1176. PubMed |
| An enzymatic fluorimetric assay for determination of N-acetylaspartate. Becker I, Eckhardt M. Anal Biochem. 2023 Apr 15;667:115083. doi: 10.1016/j.ab.2023.115083. Epub 2023 Feb 15. PubMed |
| Programmable RNA detection with CRISPR-Cas12a. Jain P, Rananaware S, Vesco E, Shoemaker G, Anekar S, Sandoval LS, Meister K, Macaluso N, Nguyen L. Res Sq. 2023 Feb 17:rs.3.rs-2549171. doi: 10.21203/rs.3.rs-2549171/v1. Preprint. PubMed |
| TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport. Canty JT, Hensley A, Aslan M, Jack A, Yildiz A. Nat Commun. 2023 Mar 13;14(1):1376. doi: 10.1038/s41467-023-36945-8. PubMed |
| Crotamine-based recombinant immunotoxin targeting HER2 for enhanced cancer cell specificity and cytotoxicity. Jang J, Nguyen MQ, Park S, Ryu D, Park H, Lee G, Kim CJ, Jang YJ, Choe H. Toxicon. 2023 Jul;230:107157. doi: 10.1016/j.toxicon.2023.107157. Epub 2023 May 15. PubMed |
| Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin. Liu N, Konuma T, Sharma R, Wang D, Zhao N, Cao L, Ju Y, Liu D, Wang S, Bosch A, Sun Y, Zhang S, Ji D, Nagatoishi S, Suzuki N, Kikuchi M, Wakamori M, Zhao C, Ren C, Zhou TJ, Xu Y, Meslamani J, Fu S, Umehara T, Tsumoto K, Akashi S, Zeng L, Roeder RG, Walsh MJ, Zhang Q, Zhou MM. Mol Cell. 2023 Jul 6;83(13):2206-2221.e11. doi: 10.1016/j.molcel.2023.05.022. Epub 2023 Jun 12. PubMed |
| Integrated regulation of tubulin tyrosination and microtubule stability by human alpha-tubulin isotypes. Fu G, Yan S, Khoo CJ, Chao VC, Liu Z, Mukhi M, Hervas R, Li XD, Ti SC. Cell Rep. 2023 Jun 27;42(6):112653. doi: 10.1016/j.celrep.2023.112653. Epub 2023 Jun 22. PubMed |
| Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR. He Y, Cotten ML, Yin J, Yuan Q, Tjandra N. Protein Expr Purif. 2023 Dec;212:106354. doi: 10.1016/j.pep.2023.106354. Epub 2023 Aug 18. PubMed |
| Deep mutational scanning highlights a role for cytosolic regions in Hrd1 function. Peterson BG, Hwang J, Russ JE, Schroeder JW, Freddolino L, Baldridge RD. Cell Rep. 2023 Nov 28;42(11):113451. doi: 10.1016/j.celrep.2023.113451. Epub 2023 Nov 18. PubMed |
| Dynamical control enables the formation of demixed biomolecular condensates. Lin AZ, Ruff KM, Dar F, Jalihal A, King MR, Lalmansingh JM, Posey AE, Erkamp NA, Seim I, Gladfelter AS, Pappu RV. Nat Commun. 2023 Nov 24;14(1):7678. doi: 10.1038/s41467-023-43489-4. PubMed |
| The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Vieira MFM, Hernandez G, Zhong Q, Arbesu M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazao C, Frankel G, Zanzoni A, Cordeiro TN. Commun Biol. 2024 Feb 13;7(1):179. doi: 10.1038/s42003-024-05856-9. PubMed |
| Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs. Nguyen LT, Macaluso NC, Rakestraw NR, Carman DR, Pizzano BLM, Hautamaki RC, Rananaware SR, Roberts IE, Jain PK. Cell Rep. 2024 Feb 27;43(2):113777. doi: 10.1016/j.celrep.2024.113777. Epub 2024 Feb 14. PubMed |
| Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting. Li J, Purser N, Liwocha J, Scott DC, Byers HA, Steigenberger B, Hill S, Tripathi-Giesgen I, Hinkle T, Hansen FM, Prabu JR, Radhakrishnan SK, Kirkpatrick DS, Reichermeier KM, Schulman BA, Kleiger G. Mol Cell. 2024 Apr 4;84(7):1304-1320.e16. doi: 10.1016/j.molcel.2024.01.022. Epub 2024 Feb 20. PubMed |
| Optimizing protein delivery rate from silk fibroin hydrogel using silk fibroin-mimetic peptides conjugation. Promsuk J, Manissorn J, Laomeephol C, Luckanagul JA, Methachittipan A, Tonsomboon K, Jenjob R, Yang SG, Thongnuek P, Wangkanont K. Sci Rep. 2024 Feb 23;14(1):4428. doi: 10.1038/s41598-024-53689-7. PubMed |
| The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3. Danlasky BM, Martinez M, Cassani M, Bodas D, Putnam A, Seydoux G. MicroPubl Biol. 2024 Feb 16;2024:10.17912/micropub.biology.001137. doi: 10.17912/micropub.biology.001137. eCollection 2024. PubMed |
| A temperature-inducible protein module for control of mammalian cell fate. Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ. bioRxiv [Preprint]. 2024 Feb 19:2024.02.19.581019. doi: 10.1101/2024.02.19.581019. PubMed |
| Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency. Lorton BM, Warren C, Ilyas H, Nandigrami P, Hegde S, Cahill S, Lehman SM, Shabanowitz J, Hunt DF, Fiser A, Cowburn D, Shechter D. iScience. 2024 Mar 8;27(4):109458. doi: 10.1016/j.isci.2024.109458. eCollection 2024 Apr 19. PubMed |
| Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies. Kimura-Someya T, Katsura K, Kato-Murayama M, Hosaka T, Uchikubo-Kamo T, Ihara K, Hanada K, Sato S, Murayama K, Kataoka M, Shirouzu M, Someya Y. J Virol. 2024 May 14;98(5):e0019724. doi: 10.1128/jvi.00197-24. Epub 2024 Apr 9. PubMed |
| Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1. Nashed A, Naidoo KJ. ACS Omega. 2024 Apr 5;9(15):17518-17532. doi: 10.1021/acsomega.4c00485. eCollection 2024 Apr 16. PubMed |
Modular and integrative activity reporters enhance biochemical studies in the yeast ER.
Martinusen SG, Slaton EW, Nelson SE, Pulgar MA, Besu JT, Simas CF, Denard CA.
Protein Eng Des Sel. 2024 Jan 29;37:gzae008. doi: 10.1093/protein/gzae008.
PubMed
Associated Plasmids |
| Proline Isomerization and Molten Globular Property of TgPDCD5 Secreted from Toxoplasma gondii Confers Its Regulation of Heparin Sulfate Binding. Lin GM, Yu TA, Chang CF, Hsu CH. JACS Au. 2024 Mar 20;4(5):1763-1774. doi: 10.1021/jacsau.3c00577. eCollection 2024 May 27. PubMed |
| Kunitz-type trypsin inhibitor from durian (Durio zibethinus) employs a distinct loop for trypsin inhibition. Deetanya P, Limsardsanakij K, Sabat G, Pattaradilokrat S, Chaisuekul C, Wangkanont K. Protein Sci. 2024 Dec;33(12):e5230. doi: 10.1002/pro.5230. PubMed |
| G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation. Parker DM, Tauber D, Parker R. Mol Cell. 2025 Feb 6;85(3):571-584.e7. doi: 10.1016/j.molcel.2024.11.012. Epub 2024 Dec 4. PubMed |
| AcrVIB1 inhibits CRISPR-Cas13b immunity by promoting unproductive crRNA binding accessible to RNase attack. Wandera KG, Schmelz S, Migur A, Kibe A, Lukat P, Achmedov T, Caliskan N, Blankenfeldt W, Beisel CL. Mol Cell. 2025 Mar 20;85(6):1162-1175.e7. doi: 10.1016/j.molcel.2025.01.020. Epub 2025 Feb 17. PubMed |
| Short 5' UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex. Glasner DR, Todd C, Cook B, D'Urso A, Khosla S, Estrada E, Wagner JD, Bartels MD, Ford P, Prych J, Hatch K, Yee BA, Ego KM, Liang Q, Holland SR, Case JB, Corbett KD, Diamond MS, Yeo GW, Herzik MA Jr, Van Nostrand EL, Daugherty MD. bioRxiv [Preprint]. 2025 Feb 11:2025.02.11.637299. doi: 10.1101/2025.02.11.637299. PubMed |
| Establishment of a Novel Caco-2-Based Cell Culture System for Human Sapovirus Propagation. Fukuda Y, Ishikawa A, Ishiyama R, Takai-Todaka R, Haga K, Someya Y, Kimura-Someya T, Katayama K. Genes Cells. 2025 Mar;30(2):e70007. doi: 10.1111/gtc.70007. PubMed |
| Engineering and Structural Elucidation of a Sac7d-Derived IgG Fc-Specific Affitin and Its Application for the Light-Controlled Affinity Purification of Antibodies. Veitl F, Eichinger A, Mayrhofer P, Anneser MR, Testanera M, Rauscher K, Lenz M, Skerra A. Chembiochem. 2025 Jun 3;26(11):e202500102. doi: 10.1002/cbic.202500102. Epub 2025 Apr 7. PubMed |
| PERRC: Protease Engineering with Reactant Residence Time Control. Nelson S, Gaza J, Ajayebi S, Masse R, Pho R, Scutero C, Martinusen S, Long L, Menezes A, Perez A, Denard C. bioRxiv [Preprint]. 2025 Mar 4:2025.03.02.641063. doi: 10.1101/2025.03.02.641063. PubMed |
| Reconstruction of the ancient cyanobacterial proto-circadian clock system KaiABC. Li S, Zhou Z, Wan Y, Jia X, Wang P, Wang Y, Zuo T, Cheng H, Fang X, Dong S, He J, Yang Y, Xu Y, Fu S, Wang X, Qin X, Xie Q, Xu X, Zhao Y, Liang D, Zhang P, Zhang Q, Guo J. EMBO J. 2025 May;44(10):3025-3046. doi: 10.1038/s44318-025-00425-0. Epub 2025 Apr 10. PubMed |
| PERRC: Protease Engineering with Reactant Residence Time Control. Nelson S, Gaza J, Ajayebi S, Masse R, Pho R, Scutero C, Martinusen S, Long L, Menezes A, Perez A, Denard C. ACS Synth Biol. 2025 Jun 20;14(6):2241-2253. doi: 10.1021/acssynbio.5c00154. Epub 2025 May 19. PubMed |
| Regulatory mechanisms of PP2A complex assembly driven by physicochemical differences in A-subunit isoforms. Day A, Huang W, Leonard D, O'Connor CM, Narla G, Taylor DJ. Structure. 2025 Oct 2;33(10):1688-1699.e5. doi: 10.1016/j.str.2025.06.013. Epub 2025 Jul 24. PubMed |
| A New Highly Specific and Soluble Protease for Precise Removal of N‑Terminal Purification Tags. Shams R, Regan L. ACS Omega. 2025 Jul 11;10(28):30354-30364. doi: 10.1021/acsomega.5c01764. eCollection 2025 Jul 22. PubMed |
| AsCas12a tolerates insertions in target DNA. Rananaware SR, Shoemaker GM, Pizzano BLM, Vesco EK, Sandoval LSW, Lewis JG, Bodin AP, Flannery SJ, Lange IH, Pedada D, Fang A, Antal SG, Aguilar D, Rakestraw NR, Karalkar VN, Meister KS, Nguyen LT, Jain PK. Nucleic Acids Res. 2025 Sep 5;53(17):gkaf887. doi: 10.1093/nar/gkaf887. PubMed |
| The IFIT2-IFIT3 antiviral complex targets short 5' untranslated regions on viral mRNAs for translation inhibition. Glasner DR, Todd C, Cook B, D'Urso A, Khosla S, Estrada E, Wagner JD, Bartels MD, Hung CT, Ford P, Prych J, Hatch KS, Yee BA, Ego KM, Liang Q, Holland SR, Case JB, Corbett KD, Diamond MS, Lee B, Yeo GW, Herzik MA Jr, Van Nostrand EL, Daugherty MD. Nat Microbiol. 2025 Nov;10(11):2934-2948. doi: 10.1038/s41564-025-02138-w. Epub 2025 Oct 15. PubMed |
If you have published an article using this material, please email us at [email protected] to have your article added to this page.