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Jannik Elverløv-Jakobsen Lab: Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Unpublished

Plasmids from Article

ID Plasmid Purpose  
68345LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIRA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
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68346AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPAUsed to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4
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68347AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTRAAV plasmid expressing the FlpO recombinase. with empty gRNA
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68348px330_PTEN exon 5 gRNApx330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
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68349px330_P53 exon 8 gRNApx330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
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68350px330_SMAD exon 2 gRNApx330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
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68351px330_P53 exon 7 gRNApx330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
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68352px330_SMAD exon 9 gRNApx330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
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683564xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
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683575xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpOThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
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684053xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpOThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
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