A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination.
Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD
Cell. 2017 Mar 23;169(1):108-119.e20. doi: 10.1016/j.cell.2017.03.006.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89344 | DNA-CARzeta-GFP | 2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP |
| 89345 | DNA-CARzeta-IRESpuro | 2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette |
| 89346 | DNA-CARtcr-IRESpuro | 2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette |
| 89347 | TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta | 2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences |