Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi.
Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen DT
Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x. PubMed Article
Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen DT
Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137032 | pRSET-TEV-12His | empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible) |
| 137033 | pBBA57-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12 |
| 137034 | pRevA-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12 |
| 137035 | pP13-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12 |
| 137036 | pHtrA-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12 |
| 137038 | pBB0238-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12 |
| 137039 | pBB0323-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12 |
| 137040 | pBB0405-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12 |
| 137042 | pDipA-TEV-His12 | expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12 |
| 137045 | pelB-MBP-P66-ss | E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137046 | pelB-MBP-BB0238-ss | E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137064 | pelB-MBP-RevA-ss | E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137065 | pelB-MBP-P13-ss | E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137066 | pelB-MBP-HtrA-ss | E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137067 | pelB-MBP-BB0323-ss | E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137068 | pelB-MBP-DipA-ss | E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage |
| 137069 | pUC57-BBclone1 | E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238 |
| 137070 | pCC1-4k-BBclone2 | E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1 |
| 138651 | KTD101(DE3) | KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter |