Melanoma-associated mutants within the serine-rich domain of PAK5 direct kinase activity to mitogenic pathways.
LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
Oncotarget. 2018 May 22;9(39):25386-25401. doi: 10.18632/oncotarget.25356. eCollection 2018 May 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167168 | pLenti--Puro-3xFLAG PAK5 S364L | puromycin resistant lentiviral vector for expression of PAK5 S364L |
| 167179 | pLenti-3xFLAG-Puro PAK5 V593I | puromycin resistant lentiviral vector for expression of PAK5 V593I |
| 167180 | pLenti-3xFLAG-Puro PAK5 K478R K469R | puromycin resistant lentiviral vector for expression of PAK5 K478R K469R |
| 167181 | pLenti-3xFLAG-Puro PAK5 T538N | puromycin resistant lentiviral vector for expression of PAK5 T538N |
| 167192 | pLenti-3xFLAG-Puro PAK5 M173I | puromycin resistant lentiviral vector for expression of PAK5 M173I |
| 167193 | pLenti-3xFLAG-Puro PAK5 E144K | puromycin resistant lentiviral vector for expression of PAK5 E144K |
| 167195 | pLenti-3xFLAG-Puro PAK5 S573N | puromycin resistant lentiviral vector for expression of PAK5 S573N |
| 167196 | pLenti-3xFLAG-Puro PAK5 K478R/479R E144K | puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant |
| 167197 | pLenti-3xFLAG-Puro PAK5 K478R/479R M173I | puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant |
| 167198 | pLenti-3xFLAG-Puro PAK5 K478R/479R E294K | puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant |
| 167199 | pLenti-3xFLAG-Puro PAK5 K478R/479R S364L | puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant |
| 167200 | pLenti-3xFLAG-Puro PAK5 K478R/479R D421N | puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant |