Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection.
Shukla N, Roelle SM, Suzart VG, Bruchez AM, Matreyek KA
PLoS Pathog. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715. eCollection 2021 Jul.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171588 | pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3 | Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter |
| 171594 | AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR | Strong human ACE2 abundance from Bxb1 landing pad |
| 171595 | AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR | Weak human ACE2 abundance from Bxb1 landing pad |
| 171596 | AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR | Ectodomain deleted negative control ACE2 from Bxb1 landing pad |
| 171597 | AttB _EGFP | Strong EGFP fluorescence from Bxb1 landing pad |
| 171598 | AttB_mCherry | Strong mCherry fluorescence from Bxb1 landing pad |
| 171599 | pLenti_CMV-EGFP-2A-mNeonGreen | Green lentiviral vector plasmid for pseudovirus experiments |