Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells.
Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S
J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178861 | pCMV-PB1-AG | Mammalian expression of PB1-AzamiGreen |
| 178862 | pCMV-PB1-AG-FRB | Mammalian expression of PB1-AzamiGreen-FRB |
| 178863 | pCMV-FKBP-mCherry-MCS | Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mCherry |
| 178864 | pCMV-FKBP-mTagBFP2-MCS | Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2 |
| 178865 | pCMV-FKBP-mCherry-Vav2cat | Mammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherry |
| 178866 | pCMV-FKBP-mTagBFP2-SOScat | Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2 |
| 178867 | pCMV-mCherry-FKBP-TEVp | Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242) |
| 178868 | pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA | Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA |
| 178869 | pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA | Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA |
| 178870 | pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat | Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA |
| 178871 | pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat | Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA |
| 178872 | pCAGGS-PB1-MontiRed-LOV2 | Mammalian expression of PB1-MontiRed-LOV2 |
| 178873 | pCMV-miRFP670-Zdk1-MCS | Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1 |
| 178874 | pCAGGS-PB1-MontiRed-LOV2dark | Mammalian expression of PB1-MontiRed-LOV2dark |
| 178875 | pCMV-mCherry-Zdk1-Vav2cat | Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1 |
| 178876 | pCAGGS-PB1-AG(Y63L)-LOV2 | Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2 |