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Rapid directed molecular evolution of fluorescent proteins in mammalian cells.
Babakhanova S, Jung EE, Namikawa K, Zhang H, Wang Y, Subach OM, Korzhenevskiy DA, Rakitina TV, Xiao X, Wang W, Shi J, Drobizhev M, Park D, Eisenhard L, Tang H, Koster RW, Subach FV, Boyden ES, Piatkevich KD
Protein Sci. 2022 Mar;31(3):728-751. doi: 10.1002/pro.4261. Epub 2021 Dec 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
178965pmiRFP2-Actin-C-18Expresses the fusion protein of miRFP2-Actin-C in mammalian cells
178966pmiRFP2-P2A-HO1-N1Expresses miRFP2 and HO1 in mammalian cells
178967pCMV-miRFP2-IRES-HO1-N1Expresses miRFP2 and HO1 in mammalian cells
178968pAAV-CAG-miRFP2Expresses miRFP2 in mammalian cells
178969pSF11-wmiRFP2Expresses miRFP2 in worm cells
178971pAAV-CAG-TagRFP658-P2A-GFPExpresses TagRFP658 and GFP in mammalian cells
178972pKeratin-emiRFP2-N1Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells
178973pphiLOV3-N1Expresses phiLOV3 in mammalian cells
178974pTagRFP658-N1Expresses TagRFP658 in mammalian cells
179485pSF11-wmiRFP2-T2A-HO1Expresses miRFP2 and HO1in worm cells
196109pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3
196110pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3
196111pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3
196112pTol2zmiRFP-EF1-2xCMV-EF1-mClover3mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

Antibodies from Article