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Structural snapshots of R-loop formation by a type I-C CRISPR Cascade.

O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW
Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196399pCDF_TypeIC_target_sfGFP_invivoexpresses CRISPR Cascade Type IC target sequence for in vivo assay
196400pBAD_TypeIC_Cascade_cas3_invivoexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay
196402pET28b_TypeIC_acrIF2_invivoexpresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay
196403pET28b_TypeIC_acrIC4_invivoexpresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Antibodies from Article