Enhanced single RNA imaging reveals dynamic gene expression in live animals.
Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W
Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200763 | pSX2719 | Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans. |
| 200764 | pSX2720 | Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans. |
| 200765 | pSX2739 | Expresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used. |
| 205157 | pcDNA-tdMCP-24xGCN4 | express tdMCP-24xGCN4 |
| 205158 | pcDNA-tdMCP-12xGCN4 | express tdMCP-12xGCN4 |
| 205159 | pcDNA-tdMCP-6xGCN4 | express tdMCP-6xGCN4 |
| 205160 | pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2 | express β-ACTIN-3′UTR-8xMS2 |
| 205161 | pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2 | express β-ACTIN-3′UTR-24xMS2 |
| 205162 | pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI | express scFv-GCN4-HAtag-msfGFP(V206K)-GBI |
| 205163 | pcDNA-tdMCP-msfGFP(V206K)-GBI | express tdMCP-msfGFP(V206K)-GBI |