Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery.
Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Uoselis L, Lazarou M, Hummer G, Hurley JH, Martens S
Nat Cell Biol. 2025 Jul 25. doi: 10.1038/s41556-025-01712-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223723 | pETDuet1_His-TEV-EGFP | Expression of recombinant protein for purification |
| 223724 | pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa) | Expression of recombinant protein for purification |
| 223725 | pETDuet1_6xHis-TEV-mCherry-WIPI2d | Expression of recombinant protein for purification |
| 223726 | pGEX-4T1_GST-LC3A | Expression of recombinant protein for purification |
| 223727 | pGEX-4T1_GST-LC3C | Expression of recombinant protein for purification |
| 223728 | pGEX-4T1_GST-GBRP | Expression of recombinant protein for purification |
| 223729 | pGEX-4T1_GST-GBRPL1 | Expression of recombinant protein for purification |
| 223730 | pGEX-4T1_GST-GBRPL2 | Expression of recombinant protein for purification |
| 223731 | pGenLenti V5-NIX | Stable expression of protein in mammalian cell culture |
| 223732 | pGenLenti V5-BNIP3 | Stable expression of protein in mammalian cell culture |
| 223733 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT) | Expression of recombinant protein for purification |
| 223734 | pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT) | Expression of recombinant protein for purification |
| 223735 | pCAG_mCherry-ATG13 (WT) | Expression of recombinant protein for purification |
| 223736 | pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT) | Expression of recombinant protein for purification |
| 223737 | pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT) | Expression of recombinant protein for purification |
| 223738 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR) | Expression of recombinant protein for purification |
| 223739 | pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
| 223740 | pBIG1a_GST-TEV-CK2alpha_CK2beta | Expression of recombinant protein for purification |
| 223741 | pBIG1a_GST-TEV-CK2alpha'_CK2beta | Expression of recombinant protein for purification |
| 223742 | pLIB_GST-TEV-SRC(WT) | Expression of recombinant protein for purification, codon optimized for Insect cells |
| 223743 | pLIB_GST-TEV-SRC (Y530F) | Expression of recombinant protein for purification |
| 223744 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST | Expression of recombinant protein for purification |
| 223745 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST | Expression of recombinant protein for purification |
| 223746 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1) | Expression of recombinant protein for purification |
| 223747 | pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase | Expression of recombinant protein for purification |
| 223748 | pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR) | Expression of recombinant protein for purification |
| 223749 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1) | Expression of recombinant protein for purification |
| 223750 | pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
| 223751 | pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A) | Expression of recombinant protein for purification |
| 223752 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2) | Expression of recombinant protein for purification |
| 223753 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2) | Expression of recombinant protein for purification |
| 223754 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3) | Expression of recombinant protein for purification |
| 223755 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4) | Expression of recombinant protein for purification |
| 223756 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4) | Expression of recombinant protein for purification |
| 223757 | pHAGE-FKBP-mEGFP-WIPI2 | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223758 | pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa) | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223759 | pCAG_mCherry-ATG13 HORMA (1-191aa) | Expression of recombinant protein for purification |
| 223760 | pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa) | Expression of recombinant protein for purification |
| 223761 | pGEX4T1_GST-TEV-EGFP-ATG13_IDR_191-517aa_(M196A_S197A_R199A) | Expression of recombinant protein for purification |
| 223762 | pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa) | Expression of recombinant protein for purification |
| 223763 | pETDuet1_6xHis-TEV-mCherry-WIPI3 | Expression of recombinant protein for purification |
| 223764 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST | Expression of recombinant protein for purification |
| 223765 | pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST | Expression of recombinant protein for purification |
| 223766 | pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
| 223767 | pHAGE-FKBP-mEGFP-WIPI1 | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223768 | pHAGE-FKBP-mEGFP-WIPI3 | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223769 | pHAGE-FKBP-mEGFP-WIPI4 | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223770 | pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E) | Stable expression and inducible recruitment of protein in mammalian cell culture |
| 223771 | pGenlenti_ATG13-WT | Stable expression of protein in mammalian cell culture, rescue experiments |
| 223772 | pGenlenti_ATG13-delta191-230 | Stable expression of protein in mammalian cell culture, rescue experiments |
| 223773 | pGenlenti_ATG13-delta191-205 | Stable expression of protein in mammalian cell culture, rescue experiments |
| 223774 | pGenlenti_ATG13-delta-206-230 | Stable expression of protein in mammalian cell culture, rescue experiments |
| 223775 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3) | Expression of recombinant protein for purification |
| 223776 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4) | Expression of recombinant protein for purification |
| 223777 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2) | Expression of recombinant protein for purification |
| 223778 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
| 223779 | pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA) | Expression of recombinant protein for purification |
| 223780 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A; deltaLIR1+3) | Expression of recombinant protein for purification |
| 223781 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A/I307A/V310A; deltaLIR1+4) | Expression of recombinant protein for purification |
| 223782 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2) | Expression of recombinant protein for purification |
| 223783 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2) | Expression of recombinant protein for purification |
| 223784 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4) | Expression of recombinant protein for purification |
| 223785 | pGEX4T1_GST-EGFP-ATG13_IDR_delta191-230 (231-517aa) | Expression of recombinant protein for purification |
| 223786 | pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa) | Expression of recombinant protein for purification |
| 223787 | pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa) | Expression of recombinant protein for purification |
| 223788 | pGenLenti V5-NIX (W36A/L39A; deltaLIR) | Stable expression of protein in mammalian cell culture |
| 223789 | pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2) | Stable expression of protein in mammalian cell culture |
| 223790 | pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa) | Expression of recombinant protein for purification |
| 223791 | pGEX-4T1_GST-EGFP-ATG13_IDR (191-205aa) | Expression of recombinant protein for purification |
| 223792 | pGEX-4T1_GST-EGFP-ATG13_IDR (191-230aa) | Expression of recombinant protein for purification |
| 223793 | pGEX-4T1_GST-EGFP-ATG13_IDR (206-230aa) | Expression of recombinant protein for purification |
| 223794 | pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima) | Stable expression of protein in mammalian cell culture |
| 223795 | pHAGE-FRB-Fis1 (no mt-mKeima) | Stable expression of protein in mammalian cell culture |
| 223796 | pCAG_ATG101 | Expression of recombinant protein for purification (co-expression with ATG13) |
| 223797 | pCAG_GST-TEV-EGFP-ATG13 | Expression of recombinant protein for purification |
| 223798 | pCAG_GST-TEV-WIPI1 | Expression of recombinant protein for purification |
| 223799 | pCAG_WIPI2d-TEV-GST | Expression of recombinant protein for purification |
| 223800 | pCAG_GST-TEV-WIPI3 | Expression of recombinant protein for purification |
| 223801 | pCAG_GST-TEV-WIPI4 | Expression of recombinant protein for purification |
| 227710 | pETDuet_6xHis-TEV-mCherry | Expression of recombinant protein for purification (used as control) |
| 227711 | pETDuet_6xHis-TEV-mCherry-ATG13 IDR(230-517aa) | Expression of recombinant protein for purification |
| 227712 | pETDuet_6xHis-FKBP8(1-391aa)-thrombin-GST | Expression of recombinant protein for purification |
| 227713 | pETDuet_6xHis-CCPG1(1-212aa)-thrombin-GST | Expression of recombinant protein for purification |
| 227714 | pGEX-4T1_GST-thrombin-TEX264(28-313aa) | Expression of recombinant protein for purification |
| 227715 | pGEX-4T1_GST-thrombin-FAM134C(250-466aa) | Expression of recombinant protein for purification |
| 239015 | pCAG-MBP-OSF-TEVcs-GFP-hULK1 | Expression of recombinant protein for purification |