A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy.

Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM, Sekine S, Li Y, Ward ME, Narendra DP
EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
236729	pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2	Dual sgRNA empty vector expressing BFP with Puromycin selection
236730	pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2	Dual sgRNA targeting CTRL expressing BFP with Puromycin selection
236731	pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2	Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection
236733	pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2	Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER
236734	pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2	Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER
239866	MFN2-Halo donor plasmid	endogenously tags MFN2 with Halo tag
239867	TOMM70-FKBP12-Halo donor plasmid	endogenously tags TOMM70 with Halo tag