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The COOH-Terminal Proline-Rich Region of GRP78 Is a Key Regulator of Its Cell Surface Expression and Viability of Tamoxifen-Resistant Breast Cancer Cells.

Tseng CC, Zhang P, Lee AS
Neoplasia. 2019 Aug;21(8):837-848. doi: 10.1016/j.neo.2019.05.008. Epub 2019 Jul 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
238421GST-GRP78-Full Length (pGEX4T1)Expresses GRP78 Full Length fused to the GST Tag
238422GST-GRP78-N Terminus (pGEX4T1)Expresses GRP78 N terminus fused to the GST Tag
238423GST-GRP78-C Terminus (pGEX4T1)Expresses GRP78 C Terminus fused to the GST Tag
238424GST-GRP78-Delta KDEL (pGEX4T1)Expresses GRP78 Delta KDEL fused to the GST Tag
238425GST-GRP78-Delta C11(pGEX4T1)Expresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST Tag
238426GST-GRP78-Delta C17 (pGEX4T1)Expresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST Tag
238427GST-GRP78-Delta C73 (pGEX4T1)Expresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST Tag
238428GST-GRP78-C73 (pGEX4T1)Expresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST Tag
238429FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)Expresses GRP78 Full Length without UTRs fused to the FLAG Tag
238430FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)Expresses GRP78 AAA mutant without the UTRs fused to FLAG tag
238431pcDNA3-P-SSP-Wild-TypeExpress amino acid 631-650 of Wild-type GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)
238432pcDNA3-P-SSP-AAA MutantExpress amino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)

Antibodies from Article