James Bamburg Lab: Bamburg lab plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245942 | huADF WT-mRFP | Expressing human ADF-mRFP-chimera |
| 245944 | huADF S3E | Expressing a non-actin binding form (phosphomimetic) of human ADF |
| 245952 | ADF shRNA mouse | For making other mouse ADF silencing vectors |
| 245953 | huCof S3E SSR RedTrack | Expression of inactive (phosphomimetic) cofilin S3E resistant to shRNA in cells coexpressing mRFP |
| 245955 | huCof WT SSR RedTrack | Expression of WT coifilin resistant to shRNA in cells coexpressing mRFP |
| 245956 | huCof S3A SSR RedTrack | Expression of constitutively active cofilin S3A resistant to shRNA in cells coexpressing mRFP |
| 245958 | huCof WTmRFP | Expression of cofilin-mRFP to normal levels behind a cofilin promoter |
| 245959 | MCP-huCof K22Q + rat cof shRNA | Silencing of endogenous cofilin in rat/mouse cells with replacement by human cofilin K22Q expressed with a cofilin promoter |
| 245960 | huCof null + rat cof shRNA | Silencing of endogenous cofilin in rat/mouse cells with replacement by an inactive human cofilin expressed with a cofilin promoter (control for plasmid listed above) |
| 245961 | huCof WT + rat cof shRNA | Silencing of endogenous cofilin in rat/mouse neurons with replacment by neuronal specific expression of WT human cofilin |
| 245962 | NSE-huCof K22Q + rat cof shRNA | Silencing of endogenous cofilin in rat/mouse neurons with replacment by neuronal specific expression of K22Q mutant of human cofilin |
| 245963 | LIMK1-s | Expression of inactive LIMK-1 with some dominant negative acivity and GFP from separate CMV promoter |
| 245975 | MCP-huADF WT | Expression of ADF behind a mouse cofilin promoter. |