Multiplexed, scalable analog recording of gene regulation dynamics over weeks using intracellular protein tapes.
Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
bioRxiv [Preprint]. 2025 May 10:2025.05.10.653182. doi: 10.1101/2025.05.10.653182.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239423 | pAAV-UbC-CytoTape-HA | CytoTape structural monomer for CytoTape fiber formation and elongation for cell culture applications |
| 239424 | pAAV-Arc-CytoTape-V5 | CytoTape signal monomer for recording SARE-ArcMin promoter transcriptional activity |
| 239425 | pAAV-N-RAM-CytoTape-V5 | CytoTape signal monomer for recording Npas4 protein activity |
| 239426 | pAAV-HSPA1A-CytoTape-V5 | CytoTape signal monomer for recording HSPA1A promoter transcriptional activity |
| 239428 | pAAV-Egr1-CytoTape-V5 | CytoTape signal monomer for recording Egr1 promoter transcriptional activity |
| 239429 | pAAV-c-fos-CytoTape-V5 | CytoTape signal monomer for recording c-fos promoter transcriptional activity |
| 239430 | pAAV-CRE-CytoTape-V5 | CytoTape signal monomer for recording phosphorylated CREB protein activity |
| 239616 | pAAV-UbC-CytoTape-HaloTag | CytoTape timestamp monomer for encoding time along the CytoTape fiber for cell culture applications |