Multiplexed, scalable analog recording of gene regulation dynamics over weeks using intracellular protein tapes.
Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
bioRxiv [Preprint]. 2025 May 10:2025.05.10.653182. doi: 10.1101/2025.05.10.653182.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239423 | pAAV-UbC-CytoTape-HA | CytoTape structural monomer for CytoTape fiber formation and elongation for cell culture applications |
| 239424 | pAAV-Arc-CytoTape-V5 | CytoTape signal monomer for recording SARE-ArcMin promoter transcriptional activity |
| 239425 | pAAV-N-RAM-CytoTape-V5 | CytoTape signal monomer for recording Npas4 protein activity |
| 239426 | pAAV-HSPA1A-CytoTape-V5 | CytoTape signal monomer for recording HSPA1A promoter transcriptional activity |
| 239427 | pAAV-F-RAM-CytoTape-V5 | CytoTape signal monomer for recording Fos protein activity |
| 239428 | pAAV-Egr1-CytoTape-V5 | CytoTape signal monomer for recording Egr1 promoter transcriptional activity |
| 239429 | pAAV-c-fos-CytoTape-V5 | CytoTape signal monomer for recording c-fos promoter transcriptional activity |
| 239430 | pAAV-CRE-CytoTape-V5 | CytoTape signal monomer for recording phosphorylated CREB protein activity |
| 239616 | pAAV-UbC-CytoTape-HaloTag | CytoTape timestamp monomer for encoding time along the CytoTape fiber for cell culture applications |
| 250670 | pAAV-UbC-CytoTape-vivo-HA | CytoTape-vivo structural monomer for CytoTape-vivo fiber formation and elongation for mouse brain in vivo applications |
| 250671 | pAAV-TRE3G-XRI-FLAG | Timestamp monomer for encoding time along the CytoTape-vivo fiber for mouse brain in vivo applications. |
| 250672 | pAAV-hSyn-rtTA3G-TetOn3G | Drive Tet-ON activator expression from human synapsin promoter for doxycycline-inducible transgene activation |