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CRISPR-Cas/RGN expression plasmids for Zebrafish

The Joung Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease (RGN) technology to modify nine different sites in various endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013).

The expression plasmids encoding the guide RNAs (gRNAs) used in these experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA produced from the Cas9 expression vector (pMLM3613). Additional details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions to the Genome Engineering newsgroup (which can be joined here).

Individual plasmids can be ordered via the links below:

ID Plasmid
42251 MLM3613 (Cas9 expression vector)
42241 DR299 (gRNA targeted to zebrafish gene apoea )
42242 DR336 (gRNA targeted to zebrafish gene drd3 )
42243 DR279 (gRNA targeted to zebrafish gene fh [site #1])
42244 DR302 (gRNA targeted to zebrafish gene fh [site #2])
42245 DR338 (gRNA targeted to zebrafish gene gsk3b )
42246 DR311 (gRNA targeted to zebrafish gene rgs4 )
42247 DR317 (gRNA targeted to zebrafish gene th1 )
42248 DR330 (gRNA targeted to zebrafish gene tia1l )
42249 DR333 (gRNA targeted to zebrafish gene tph1a )