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pST_116_LVL2 cam: NT-CRISPR with a single gRNA using the regular S. pyogenes Cas9 (PAM = NGG).
pST_140_LVL2 cam: NT-CRISPR with a single gRNA using the spG Cas9, which allows alternative PAM sequences (NGT, NGC, NGA).
pST_119_LVL2 cam: Backbone plasmid for the assembly of NT-CRISPR plasmids with two to five gRNAs.
pMC0x_gRNA_Pos_Ptet: Different position vectors for gRNAs. gRNAs are first integrated in position vectors, which can then be used for the assembly of multi gRNA plasmids using pST_140_LVL2.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The NT-CRISPR Plasmid Collection was a gift from Anke Becker (Addgene kit #1000000189).”