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Human Kinase Domain Constructs Kit
(Kit # 1000000094 )

Depositing Lab:  John Chodera, Nicholas Levinson, and Markus Seeliger

These kinase plasmids were developed through a large-scale expression screen with the goal of generating a library of His-tagged human kinase constructs that express well in a simple automated bacterial expression system.

The kit contains 68 human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression. Lambda phosphatase enhances bacterial expression of Ser/Thr kinases while YopH (residues 164-468) enhances expression of Tyr kinases.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

The construction and expression screening of these plasmids are described in the following preprint:

An open library of human kinase domain constructs for automated bacterial expression. Parton DL, Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger M, Levinson NM, Chodera JD. bioRxiv Preprint.

Description

This kit consists of constructs containing His-tagged human kinases that have been optimized for automated bacterial expression. Constructs were generated to be co-expressed with a phosphatase (YopH or lambda) in order to enhance yield.

kinome_expression_tree.png

Phylogenetic tree representing kinase domain expression. Authors note that the image was created using KinMap.

Kit Documentation

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Human Kinase Domain Constructs Kit for Automated Bacterial Expression was a gift from John Chodera (Addgene kit #1000000094).”

For your Reference section:

An open library of human kinase domain constructs for automated bacterial expression. Parton DL, Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger M, Levinson NM, Chodera JD.

Human Kinase Domain Constructs for Automated Bacterial Expression - #1000000094

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 CDK16_HUMAN_D0
Ampicillin
A / 2 KCC1G_HUMAN_D0
Ampicillin
A / 3 KAPCA_HUMAN_D0
Ampicillin
A / 4 STK3_HUMAN_D0
Ampicillin
A / 5 STK24_HUMAN_D0
Ampicillin
A / 6 KCC2D_HUMAN_D0
Ampicillin
A / 7 GAK_HUMAN_D0
Ampicillin
A / 8 PMYT1_HUMAN_D0
Ampicillin
A / 9 HASP_HUMAN_D0
Ampicillin
A / 10 CDKL1_HUMAN_D0
Ampicillin
A / 11 BMPR2_HUMAN_D0
Ampicillin
A / 12 KC1G1_HUMAN_D0
Ampicillin
B / 1 DYR1A_HUMAN_D0
Ampicillin
B / 2 MK01_HUMAN_D0
Ampicillin
B / 3 VRK3_HUMAN_D0
Ampicillin
B / 4 VRK2_HUMAN_D0
Ampicillin
B / 5 KCC2A_HUMAN_D0
Ampicillin
B / 6 CSKP_HUMAN_D0
Ampicillin
B / 7 STK16_HUMAN_D0
Ampicillin
B / 8 STK10_HUMAN_D0
Ampicillin
B / 9 FGFR1_HUMAN_D0
Ampicillin
B / 10 VGFR1_HUMAN_D0
Ampicillin
B / 11 KC1E_HUMAN_D0
Ampicillin
B / 12 SRC_HUMAN_D0
Ampicillin
C / 1 KC1D_HUMAN_D0
Ampicillin
C / 2 MERTK_HUMAN_D0
Ampicillin
C / 3 FGFR3_HUMAN_D0
Ampicillin
C / 4 EPHB1_HUMAN_D0
Ampicillin
C / 5 ABL1_HUMAN_D0
Ampicillin
C / 6 EPHB2_HUMAN_D0
Ampicillin
C / 7 MK08_HUMAN_D0
Ampicillin
C / 8 EPHB3_HUMAN_D0
Ampicillin
C / 9 M3K5_HUMAN_D0
Ampicillin
C / 10 E2AK2_HUMAN_D0
Ampicillin
C / 11 CSK22_HUMAN_D0
Ampicillin
C / 12 DAPK1_HUMAN_D0
Ampicillin
D / 1 PAK7_HUMAN_D0
Ampicillin
D / 2 SLK_HUMAN_D0
Ampicillin
D / 3 KCC4_HUMAN_D0
Ampicillin
D / 4 KCC2G_HUMAN_D0
Ampicillin
D / 5 KC1G3_HUMAN_D0
Ampicillin
D / 6 CSK21_HUMAN_D0
Ampicillin
D / 7 NEK7_HUMAN_D0
Ampicillin
D / 8 CHK2_HUMAN_D0
Ampicillin
D / 9 PAK4_HUMAN_D0
Ampicillin
D / 10 MK11_HUMAN_D0
Ampicillin
D / 11 NEK1_HUMAN_D0
Ampicillin
D / 12 KS6A1_HUMAN_D1
Ampicillin
E / 1 MARK3_HUMAN_D0
Ampicillin
E / 2 MK14_HUMAN_D0
Ampicillin
E / 3 MAPK3_HUMAN_D0
Ampicillin
E / 4 VRK1_HUMAN_D0
Ampicillin
E / 5 PAK6_HUMAN_D0
Ampicillin
E / 6 MK03_HUMAN_D0
Ampicillin
E / 7 EPHA3_HUMAN_D0
Ampicillin
E / 8 FES_HUMAN_D0
Ampicillin
E / 9 MK13_HUMAN_D0
Ampicillin
E / 10 DMPK_HUMAN_D0
Ampicillin
E / 11 PHKG2_HUMAN_D0
Ampicillin
E / 12 CSK_HUMAN_D0
Ampicillin
F / 1 CDK2_HUMAN_D0
Ampicillin
F / 2 DAPK3_HUMAN_D0
Ampicillin
F / 3 DYRK2_HUMAN_D0
Ampicillin
F / 4 KCC2B_HUMAN_D0
Ampicillin
F / 5 AURKA_HUMAN_D0
Ampicillin
F / 6 FAK2_HUMAN_D0
Ampicillin
F / 7 KC1G2_HUMAN_D0
Ampicillin
F / 8 AAPK2_HUMAN_D0
Ampicillin
F / 9 YopH (residues 164-468)
Spectinomycin
F / 10 Lambda Phosphatase
Spectinomycin
Data calculated @ 2017-12-16

Kit Plate Map - #1000000094

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