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Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)
(Kit # 1000000175 )

Depositing Lab:   Adam Cohen

Plasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. PubMed PMID: 32753572

Description

The Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) plasmid kit is comprised of 19 lentiviral plasmids for expression of diverse fluorescent sensors of physiological parameters in cells and 16 entry vectors for cloning applications.

The following sensors are included in this kit:

  • Plasmid membrane voltage sensor (QuasAr2-mOrange2) and channelrhodopsin (CheRiff-eGFP)
  • Cytosolic, mitochondrial, and endoplasmid reticulum calcium sensors (GCaMP6F, Mitycam, and GCaMPer), superecliptic pHIuorins, ratiometric pHIuorins, and glutathione redox sensors (Grx1-roGFP2, mito-roGFP2-Grx1, and Grx1-roGFP1-ie_ER)
  • Cytosolic chloride (ClopHensorN), Peredox NADH/NAD+, and PercevalHR ATP/ADP sensors
  • Cytosolic (roGFP2-Orp1) and mitochondrial (mito-roGFP2-Orp1) oxidation sensors
MOSAIC array.png

Array of islands of HEK cells, each expressing a fluorescent reporter of a distinct physiological modality.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) was a gift from Adam Cohen (Addgene kit #1000000175).”

For your Reference section:

Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. PubMed PMID: 32753572

Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) - #1000000175

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
A / 1 pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Ampicillin
A / 2 pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)
Ampicillin
A / 3 pMOS008: GCaMP6F calcium sensor (cytosolic)
Ampicillin
A / 4 pMOS028: Mitycam calcium sensor (mitochondrial)
Ampicillin
A / 5 pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)
Ampicillin
A / 6 pMOS019: ClopHensorN chloride sensor (cytosolic)
Ampicillin
A / 7 pMOS005: superecliptic pHluorin (cytosolic)
Ampicillin
A / 8 pMOS011: superecliptic pHluorin (mitochondrial)
Ampicillin
A / 9 pMOS031: superecliptic pHluorin (endoplasmic reticulum)
Ampicillin
A / 10 pMOS017: ratiometric pHluorin (cytosolic)
Ampicillin
A / 11 pMOS018: ratiometric pHluorin (mitchondrial)
Ampicillin
A / 12 pMOS030: ratiometric pHluorin (endoplasmic reticulum)
Ampicillin
B / 1 pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Ampicillin
B / 2 pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Ampicillin
B / 3 pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Ampicillin
B / 4 pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Ampicillin
B / 5 pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Ampicillin
B / 6 pMOS023: Peredox NADH/NAD+ sensor (cytosolic)
Ampicillin
B / 7 pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
Ampicillin
B / 8 pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Kanamycin
B / 9 pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)
Kanamycin
B / 10 pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)
Kanamycin
B / 11 pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)
Kanamycin
B / 12 pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)
Kanamycin
C / 1 pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)
Kanamycin
C / 2 pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)
Kanamycin
C / 3 pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)
Kanamycin
C / 4 pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)
Kanamycin
C / 5 pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)
Kanamycin
C / 6 pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Kanamycin
C / 7 pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Kanamycin
C / 8 pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Kanamycin
C / 9 pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Kanamycin
C / 10 pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Kanamycin
C / 11 pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)
Kanamycin
Data calculated @ 2021-08-02

Kit Plate Map - #1000000175

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