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pDGE Dicot Genome Editing Kit
(Kit # 1000000084 )

Depositing Lab:   Johannes Stuttmann

Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. [Epub ahead of print] PubMed PMID: 27579989.

Description

This collection was designed for the simple Golden Gate cloning-based assembly of constructs for genome editing in dicotyledonous plants. The collection consists of “genome editing recipient” and “sgRNA shuttle” vectors, respectively. Recipient vectors are Agrobacterium-compatible, and contain a Cas9 expression cassette, a plant-selectable marker and a /ccdB/ cassette as a “dummy”. Shuttle vectors allow the generation of sgRNA transcriptional units from annealed oligonucleotides. sgRNA transcriptional units are then mobilized into recipient vectors, replacing the /ccdB/ dummy.

The Modular Cloning Toolkit (Addgene #1000000044), Modular Cloning Plant Parts (Addgene #1000000047) and hCas9 modules (Addgene #49771 and #49770) provided by Sylvestre Marillonnet, Nicola Patron and Sophien Kamoun, respectively, were used in construction of pDGE/pJOG plasmids.

plasmid summary scheme
Summary of plasmid design schemes.

Depositor-provided Files

The Stuttmann Lab has provided a manual that includes information about:

  • Detailed vector descriptions and architecture
  • Selection and design for gRNAs
  • Cloning protocol
Icon Stuttmann - Dicot GE Kit Manual (458.3 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The pDGE Dicot Genome Editing Kit was a gift from Johannes Stuttmann (Addgene kit # 1000000084).”

For your Reference section:

Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. [Epub ahead of print] PubMed PMID: 27579989.

pDGE Dicot Genome Editing Kit - #1000000084

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol and Ampicillin
Chloramphenicol and Spectinomycin
Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 pDGE5
Chloramphenicol and Ampicillin
A / 2 pDGE7
Chloramphenicol and Ampicillin
A / 3 pDGE8
Chloramphenicol and Ampicillin
A / 4 pDGE9
Chloramphenicol and Ampicillin
A / 5 pDGE10
Chloramphenicol and Ampicillin
A / 6 pDGE11
Chloramphenicol and Ampicillin
A / 7 pDGE12
Chloramphenicol and Ampicillin
A / 8 pDGE13
Chloramphenicol and Ampicillin
A / 9 pDGE14
Chloramphenicol and Ampicillin
A / 10 pDGE15
Chloramphenicol and Ampicillin
A / 11 pDGE6
Chloramphenicol and Ampicillin
A / 12 pJOG294
Chloramphenicol and Ampicillin
B / 1 pDGE1
Chloramphenicol and Spectinomycin
B / 2 pDGE2
Chloramphenicol and Spectinomycin
B / 3 pDGE3
Chloramphenicol and Spectinomycin
B / 4 pDGE4
Chloramphenicol and Spectinomycin
B / 5 pDGE62
Chloramphenicol and Spectinomycin
B / 6 pDGE63
Chloramphenicol and Spectinomycin
B / 7 pDGE64
Chloramphenicol and Spectinomycin
B / 8 pDGE65
Chloramphenicol and Spectinomycin
B / 9 pDGE144
Chloramphenicol and Spectinomycin
B / 10 pDGE146
Chloramphenicol and Spectinomycin
B / 11 pDGE148
Chloramphenicol and Spectinomycin
B / 12 pDGE151
Chloramphenicol and Spectinomycin
C / 1 pDGE160
Chloramphenicol and Spectinomycin
C / 2 pDGE161
Chloramphenicol and Spectinomycin
C / 3 pDGE164
Chloramphenicol and Spectinomycin
C / 4 pDGE165
Chloramphenicol and Spectinomycin
C / 5 pDGE166
Chloramphenicol and Spectinomycin
C / 6 pDGE167
Chloramphenicol and Spectinomycin
C / 7 pDGE168
Chloramphenicol and Spectinomycin
C / 8 pDGE169
Chloramphenicol and Spectinomycin
C / 9 pDGE76
Chloramphenicol and Spectinomycin
C / 10 pDGE77
Chloramphenicol and Spectinomycin
C / 11 pDGE78
Chloramphenicol and Spectinomycin
C / 12 pDGE79
Chloramphenicol and Spectinomycin
D / 1 pJOG250
Chloramphenicol and Spectinomycin
D / 2 pJOG251
Chloramphenicol and Spectinomycin
D / 3 pJOG252
Chloramphenicol and Spectinomycin
D / 4 pJOG253
Chloramphenicol and Spectinomycin
D / 5 pJOG292
Spectinomycin
Data calculated @ 2017-11-25

Kit Plate Map - #1000000084

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