CROPseq-iT7 Human CRISPR Knockout Pooled Library (Brunello)
(Pooled Library #223064)

• Purpose:

  This library combines gRNAs from the Brunello Human CRISPR Knockout Pooled Library (Addgene #73178) with an inverted T7 promoter downstream of the guide cassette to enable genome-scale optical perturbation screening. This method uses fluorescence microscopy for optical phenotyping in living cells and Nuclear In-Situ Sequencing (NIS-Seq) to identify individual gRNAs in the same cells. In principle, any phenotype observable by microscopy and any nucleated cell type is thus compatible with CRISPR perturbation screening.

• Vector Backbone:

  CROPseq-iT7 (Plasmid #211699) - does not express Cas9

• Depositing Labs: Jonathan Schmid-Burgk
• Publication: Fandrey et al. Nat Biotechnol. 2024 Dec 19. doi: 10.1038/s41587-024-02516-5.

Library Details

• Species: Human
• Genes targeted: 19,114
• gRNAs: 76,441
• Controls: 1,000
• Lentiviral generation: 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (PDF, 188 KB)
• Depositor Data:
  • List of gRNA sequences (XLSX, 1.8 MB)
  • Library Prepresentation Plots (PDF, 63 KB)
• Scripts:
  • Optical Screening Analysis Suite

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.