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Adli Human Druggable Library
(Pooled Library #256464)

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  • Purpose

    The Adli Human Druggable Library is a custom-designed sgRNA library that targets ~1,400 genes whose protein products or their homologs are targetable with small molecule chemicals. “Druggable” genes were chosen based on literature review and published gene-drug interaction databases.

  • Vector Backbone

    lentiGuide-Puro (Plasmid #52963) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 256464 Adli Human Druggable Library† 1 Pending
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with any Cas9 plasmid that contains a selection marker other than puromycin such as lentiCas9-Blast (Plasmid #52962).

Library Details

  • Species
    Human
  • Genes targeted
    1,422
  • gRNAs
    10,454
  • Controls
    500 non-targeting gRNAs
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    The Adli Human Druggable Library was a gift from Mazhar Adli (Addgene #256464 ; http://n2t.net/addgene:256464 ; RRID:Addgene_256464)

  • For your References section:

    Druggable genome CRISPR screening identifies the KEAP1/NRF2 axis as a mediator of PD-L1 expression. Seker-Polat F, Rogozinska M, Ban Y, Abdula F, Buyukcelebi K, Xie P, Fan J, Abbaszadeh N, Kingham Y, Paylakhi SZ, Zhang B, Adli M. Commun Biol. 2025 Nov 19;8(1):1610. doi: 10.1038/s42003-025-08983-z. PubMed 41261174
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