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Autodisplay Toolbox Libraries
(Pooled Library #235136, #235137, #235138)

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  • Purpose

    The Autodisplay Toolbox (ATB) is a collection of plasmids (pATB) that enable the display of recombinant proteins at the surface of Gram-negative bacterial cells (autodisplay). Based on a classical (EhaA) and an inverse (YeeJ) autotransporter protein, variants were constructed with different signal peptides, linkers, and adjacent regions. To account for different expression needs, gene expression was set under control of three different promoters (ParaBAD, PrhaBAD, and PRox306) resulting in three separate plasmid libraries (one library per promoter, 27 plasmids per library, 81 pATB in total). The pooled plasmids contain a passenger domain to be cloned out and replaced with the insert of interest. The ATB can then be used to screen for optimized bacterial surface display for a protein of interest.

    Individual library plasmids are also available.

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 235136 pATB_1yz 1 $451 Add to Cart
Pooled Library 235137 pATB_2yz 1 $451 Add to Cart
Pooled Library 235138 pATB_3yz 1 $451 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Number of plasmids
    27 plasmids per library
  • Size of inserts
    1,719 bp

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pATB_1yz library was a gift from Joachim Jose (Addgene #235136 ; http://n2t.net/addgene:235136 ; RRID:Addgene_235136)
    pATB_2yz library was a gift from Joachim Jose (Addgene #235137 ; http://n2t.net/addgene:235137 ; RRID:Addgene_235137)
    pATB_3yz library was a gift from Joachim Jose (Addgene #235138 ; http://n2t.net/addgene:235138 ; RRID:Addgene_235138)

  • For your References section:

    A plasmid toolbox for the easy autodisplay of recombinant proteins and its optimization. Furtmann C, Röhe P, Gesing K, Kuss H, Lenz F, Jose J. Commun Biol. 2026 May 21;9(1):694. doi: 10.1038/s42003-026-10324-7. PubMed 42168324
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