TUNEYALI Library
(Pooled Library #217744)

• Purpose:

  This CRISPR-based library is designed to target 56 transcription factors in Yarrowia lipolytica, enabling the knockout or modulation of expression levels for these factors. By replacing the native promoters of these transcription factors with alternatives of varying strengths, the library facilitates precise control over their expression in Y. lipolytica. The library can be used for knockout, activation, or inhibition.

• Vector Backbone:

  pCfB3405 (Plasmid #106166) - does not express Cas9

• Depositing Labs: Irina Borodina
• Publication: Regulating transcription factors in Yarrowia lipolytica (unpublished)

Library Details

• Species: Yarrowia lipolytica
• Genes targeted: 56 transcription factors
• gRNAs: 1 gRNA per gene

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 25 ng/µL

Resource Information

• Protocols:
  • Library Amplification (DOCX, 15 KB)
  • Verified by Nanopore sequencing using the SQK-RBK114.96 kit
• Depositor Data:
  • List of gRNAs (XLSX, 12 KB)
  • NGS Read Counts (CSV, 12 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Figure 1: A CRISPR-Cas9-based method, TUNEYALI, designed for high-throughput gene expression tuning in the widely used industrial yeast Y. lipolytica. The TUNEYALI library facilitates the optimization of Y. lipolytica by targeting 56 transcription factors (TFs) and modulating their expression levels. Using a high-throughput screening platform, strains with optimal performance can be efficiently identified.