Camargo Lab LARRY Barcode Library Version 1
(Pooled Library #140024)
The lineage and RNA recovery (LARRY) library clonally tags cells with DNA barcodes. Reading these barcodes with single cell sequencing allows for reconstruction of genome-wide transcriptional trajectories from differentiating cell populations.
Each barcode is a 40-mer consisting of 28 random and 11 constant nucleotide bases with the structure:
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||140024||pLARRY-EGFP-bc(v1) Pooled Library||1||$500||Add to Cart|
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
The depositing lab provides a script for comparing demultiplexed replicate reactions to evaluate library diversity.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LARRY Barcode Version 1 library was a gift from Fernando Camargo (Addgene #140024)
For your References section:Lineage tracing on transcriptional landscapes links state to fate during differentiation. Weinreb C, Rodriguez-Fraticelli A, Camargo FD, Klein AM Science. 2020 Jan 23. pii: science.aaw3381. doi: 10.1126/science.aaw3381. PubMed 31974159