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Camargo Lab LARRY Barcode Library Version 1
(Pooled Library #140024)

  • Purpose

    The lineage and RNA recovery (LARRY) library clonally tags cells with DNA barcodes. Reading these barcodes with single cell sequencing allows for reconstruction of genome-wide transcriptional trajectories from differentiating cell populations.

    Each barcode is a 40-mer consisting of 28 random and 11 constant nucleotide bases with the structure:

  • Vector Backbone


Item Catalog # Description Quantity Price (USD)
Pooled Library 140024 pLARRY-EGFP-bc(v1) Pooled Library 1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Barcode diversity
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Lineage and RNA recovery barcoding library
  • Lineage and RNA recovery barcoding library

The depositing lab provides a script for comparing demultiplexed replicate reactions (Link opens in a new window) to evaluate library diversity.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARRY Barcode Version 1 library was a gift from Fernando Camargo (Addgene #140024)
  • For your References section:

    Lineage tracing on transcriptional landscapes links state to fate during differentiation. Weinreb C, Rodriguez-Fraticelli A, Camargo FD, Klein AM Science. 2020 Jan 23. pii: science.aaw3381. doi: 10.1126/science.aaw3381. PubMed 31974159