Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Camargo Lab LARRY Barcode Library Version 1
(Pooled Library #140024)

  • Purpose

    The lineage and RNA recovery (LARRY) library clonally tags cells with DNA barcodes. Reading these barcodes with single cell sequencing allows for reconstruction of genome-wide transcriptional trajectories from differentiating cell populations.

    Each barcode is a 40-mer consisting of 28 random and 11 constant nucleotide bases with the structure:
    NNNNTGNNNNCANNNNACNNNNGANNNNGTNNNNAGNNNN

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 140024 pLARRY-EGFP-bc(v1) Pooled Library 1 $500 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Barcode diversity
    245,979
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

Lineage and RNA recovery barcoding library
  • Lineage and RNA recovery barcoding library

The depositing lab provides a script for comparing demultiplexed replicate reactions to evaluate library diversity.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARRY Barcode Version 1 library was a gift from Fernando Camargo (Addgene #140024)
  • For your References section:

    Lineage tracing on transcriptional landscapes links state to fate during differentiation. Weinreb C, Rodriguez-Fraticelli A, Camargo FD, Klein AM Science. 2020 Jan 23. pii: science.aaw3381. doi: 10.1126/science.aaw3381. PubMed 31974159