Moffat Lab - Barcode Libraries
(Pooled Libraries #179851, #179852)
This lentiviral barcoding library consists of 106 unique barcodes. Each barcode contains a 12 bp degenerate region followed by two static bases (either C or G) and a unique 4 bp library code.
The library was constructed by cloning the barcodes into a third generation lentiviral vector containing both a Puromycin selection cassette and the ZsGreen fluorescent marker.
Cellular barcoding can be used to trace clonal dynamics of individual cells using stable, genetically distinct sequences inherited by all progeny.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||179851||BCL0 Barcode pooled library||1||$640||Add to Cart|
|Pooled Library||179852||BCL1 Barcode pooled library||1||$640||Add to Cart|
Number of barcodes>106
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
Both are high diversity libraries identifiable by a unique 4 bp library code: BCL0 = ACGT; BLC1= CCAA. Either library can be used for an individual experiment.
The libraries have also been used and published in Chang et al. Cancer Discov. 2021 Dec 15. doi: 10.1158/2159-8290.CD-20-1265 (preprint available on bioRxiv: doi.org/10.1101/2020.08.28.273029).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:The Barcode Library was a gift from Jason Moffat (Addgene # )
For your References section:Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive Chemotherapy. Rehman SK, Haynes J, Collignon E, Brown KR, Wang Y, Nixon AML, Bruce JP, Wintersinger JA, Singh Mer A, Lo EBL, Leung C, Lima-Fernandes E, Pedley NM, Soares F, McGibbon S, He HH, Pollet A, Pugh TJ, Haibe-Kains B, Morris Q, Ramalho-Santos M, Goyal S, Moffat J, O'Brien CA. Cell. 2021 Jan 7;184(1):226-242.e21. doi: 10.1016/j.cell.2020.11.018. PubMed 33417860