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Moffat Lab - Barcode Libraries
(Pooled Libraries #179851, #179852)

  • Purpose

    This lentiviral barcoding library consists of 106 unique barcodes. Each barcode contains a 12 bp degenerate region followed by two static bases (either C or G) and a unique 4 bp library code.

    The library was constructed by cloning the barcodes into a third generation lentiviral vector containing both a Puromycin selection cassette and the ZsGreen fluorescent marker.

    Cellular barcoding can be used to trace clonal dynamics of individual cells using stable, genetically distinct sequences inherited by all progeny.

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 179851 BCL0 Barcode pooled library 1 $600 Add to Cart
Pooled Library 179852 BCL1 Barcode pooled library 1 $600 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Number of barcodes
    >106
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼40 µL
  • Concentration
    50 ng/µL

Depositor Comments

Both are high diversity libraries identifiable by a unique 4 bp library code: BCL0 = ACGT; BLC1= CCAA. Either library can be used for an individual experiment.

The libraries have also been used and published in Chang et al. Cancer Discov. 2021 Dec 15. doi: 10.1158/2159-8290.CD-20-1265 (preprint available on bioRxiv: doi.org/10.1101/2020.08.28.273029).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    The Barcode Library was a gift from Jason Moffat (Addgene # )
  • For your References section:

    Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive Chemotherapy. Rehman SK, Haynes J, Collignon E, Brown KR, Wang Y, Nixon AML, Bruce JP, Wintersinger JA, Singh Mer A, Lo EBL, Leung C, Lima-Fernandes E, Pedley NM, Soares F, McGibbon S, He HH, Pollet A, Pugh TJ, Haibe-Kains B, Morris Q, Ramalho-Santos M, Goyal S, Moffat J, O'Brien CA. Cell. 2021 Jan 7;184(1):226-242.e21. doi: 10.1016/j.cell.2020.11.018. PubMed 33417860