(Pooled Library #62217)
Note: This library has been replaced by an updated version (see Depositor Comments below).
Human genome-scale CRISPRi library for inhibition of gene transcription. Expresses a gRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.
- Vector Backbone
pSICO derivative- does not express Cas9
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:CRISPRi library was a gift from Jonathan Weissman (Addgene #62217)
For your References section:Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation . Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9. 10.1016/j.cell.2014.09.029PubMed 25307932