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Human CRISPR lncRNA Activation Pooled Library (SAM - 3 plasmid system)
(Pooled Library #1000000106)

  • Purpose

    This human CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled library uses an engineered protein complex for the transcriptional activation of lncRNAs.

    This library consists of three components which are all provided:

    1. A nucleolytically inactive Cas9-VP64 fusion (Addgene plasmid # 61425)
    2. A gRNA incorporating two MS2 RNA aptamers at the tetraloop, stem-loop 2, and EF1a-zeo resistance marker (present in the library)
    3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein (lentiMPH v2) (Addgene plasmid #89308)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000106 Zeocin resistant gRNA pooled library + 2 helper plasmids 1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid IS included with this item.

Library Details

  • Species
    Human
  • Genes targeted
    10,504 transcriptional start sites (TSS)
  • gRNAs
    96,458
  • Controls
    500
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

You will also receive bacterial stabs of the lentiMPH plasmid (Addgene #89308) and dCas9-VP64-Blast (Addgene #61425). Please do NOT order these plasmids in addition to this library. These plasmids are SHIPPED SEPARATELY at ambient temperature.

Resource Information

Depositor Comments

This library targets 10,504 unique lncRNA transcriptional start sites (>50 bp apart) annotated in RefSeq and Cabili catalogs (∼10 sgRNAs per lncRNA).

Zhang lab SAM library logo

For additional information, protocols and an activator sgRNA design tool, visit the Zhang Lab SAM website: http://sam.genome-engineering.org/

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Human CRISPR 3-plasmid lncRNA activation pooled library (SAM) was a gift from Feng Zhang (Addgene # 1000000106)
  • For your References section:

    Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Joung J, Engreitz JM, Konermann S, Abudayyeh OO, Verdine VK, Aguet F, Gootenberg JS, Sanjana NE, Wright JB, Fulco CP, Tseng YY, Yoon CH, Boehm JS, Lander ES, Zhang F. Nature. 2017, Aug 11. doi: 10.1038/nature23451. PubMed 28792927