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173 Results for:  42230

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Plasmids, Strains, and Pooled Libraries 1–20 of 173

  1. Plasmid
    #42230
    Depositor
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    None
  2. Plasmid
    #114689
    Depositor
    Purpose
    sgRNA targeting DHC's C-terminal locus
    Insert
    DHC-C sgRNA
    Use
    Tags
    None
    Expression
    Mutation
    None
  3. Plasmid
    #114691
    Depositor
    Purpose
    sgRNA for p150's C-terminal locus
    Insert
    p150 sgRNA
    Use
    Tags
    None
    Expression
    Mutation
    None
  4. Plasmid
    #114693
    Depositor
    Purpose
    sgRNA for DHC's N-terminal locus
    Insert
    DHC-N sgRNA
    Use
    Tags
    None
    Expression
    Mutation
    None
  5. Plasmid
    #114687
    Depositor
    Purpose
    sgRNA targeting NuMA's C-terminal locus
    Insert
    NuMA sgRNA
    Use
    Tags
    None
    Expression
    Mutation
    None
  6. Plasmid
    #114715
    Depositor
    Purpose
    sgRNA for LGN's N-terminal locus
    Insert
    LGN sgRNA
    Use
    Tags
    None
    Expression
    Mutation
    None
  7. pMIA3 Empty backbone
    Plasmid
    #109399
    Depositor
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Use
    CRISPR
    Tags
    mRuby2, mtp53dn
    Expression
    Mammalian
    Mutation
    None
  8. Plasmid
    #69992
    Depositor
    Purpose
    The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.
    Insert
    gRNA that targets near LoxP sites
    Use
    Tags
    None
    Expression
    Mutation
    None
  9. Plasmid
    #92144
    Depositor
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus
    Insert
    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  10. Plasmid
    #68809
    Depositor
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.
    Insert
    sgRNA mouse Dicer1 Mouse
    Use
    Mouse Targeting, CRISPR
    Tags
    None
    Expression
    Mammalian , Bacterial
    Mutation
    None
  11. Plasmid
    #107550
    Depositor
    Purpose
    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
    Insert
    Humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    M495V/Y515N/K526E/R661Q
  12. Plasmid
    #68808
    Depositor
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of
    Insert
    sgRNA mouse Dicer1 Mouse
    Use
    Mouse Targeting, CRISPR
    Tags
    None
    Expression
    Mammalian , Bacterial
    Mutation
    None
  13. Plasmid
    #69801
    Depositor
    Purpose
    Used to make mitf knockout porcine cell line
    Insert
    mitf-sgRNA Porcine
    Insert
    SpCas9 Synthetic
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  14. Plasmid
    #85041
    Depositor
    Purpose
    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.
    Insert
    loxP-stop-loxP P1 phage, SV40
    Use
    CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  15. Plasmid
    #68807
    Depositor
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.
    Insert
    sgRNA mouse Dicer1 Mouse
    Use
    Mouse Targeting, CRISPR
    Tags
    None
    Expression
    Mammalian , Bacterial
    Mutation
    None
  16. Plasmid
    #64323
    Depositor
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide
    Insert
    Cas9 Streptococcus pyogenes
    Insert
    sgRNA cassette
    Use
    CRISPR
    Tags
    3xFLAG, NLS, T2A-EBFP2
    Expression
    Mammalian
    Mutation
    None
  17. Plasmid
    #58693
    Depositor
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein Human
    Use
    AAV, CRISPR
    Tags
    3xFlag, NLS, GyrA Nsplit Intein
    Expression
    Mammalian
    Mutation
    None
  18. Plasmid
    #64324
    Depositor
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Insert
    Cas9 Streptococcus pyogenes
    Insert
    sgRNA cassette
    Use
    CRISPR
    Tags
    3xFLAG, NLS, T2A-mCherry
    Expression
    Mammalian
    Mutation
    None
  19. Plasmid
    #58694
    Depositor
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein Human
    Use
    AAV, CRISPR
    Tags
    NLS, GyrA Csplit Intein
    Expression
    Mammalian
    Mutation
    None
  20. Plasmid
    #97083
    Depositor
    Purpose
    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform
    Insert
    sgRNA NEAT1m_v1 Human
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
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